TY  - JOUR
AU  - Kaczmarek, Isabell
AU  - Wower, Isabel
AU  - Ettig, Katja
AU  - Kuhn, Christina Katharina
AU  - Kraft, Robert
AU  - Landgraf, Kathrin
AU  - Körner, Antje
AU  - Schöneberg, Torsten
AU  - Horn, Susanne
AU  - Thor, Doreen
TI  - Identifying G protein-coupled receptors involved in adipose tissue function using the innovative RNA-seq database FATTLAS
JO  - iScience
VL  - 26
IS  - 10
SN  - 2589-0042
CY  - St. Louis
PB  - Elsevier
M1  - DKFZ-2023-01936
SP  - 107841
PY  - 2023
AB  - G protein-coupled receptors (GPCRs) modulate the function of adipose tissue (AT) in general and of adipocytes, specifically. Although it is well-established that GPCRs are widely expressed in AT, their repertoire as well as their regulation and function in (patho)physiological conditions (e.g., obesity) is not fullyresolved. Here, we established FATTLAS, an interactive public database, for improved access and analysisof RNA-seq data of mouse and human AT. After extracting the GPCRome of non-obese and obese individuals, highly expressed and differentially regulated GPCRs were identified. Exemplarily, we describe fourreceptors (GPR146, MRGPRF, FZD5, PTGER2) and analyzed their functions in a (pre)adipocyte cell model.Besides all receptors being involved in adipogenesis, MRGPRF is essential for adipocyte viability and regulates cAMP levels, while GPR146 modulates adipocyte lipolysis via constitutive activation of Gi proteins.Taken together, by implementing and using FATTLAS we describe four hitherto unrecognized GPCRsassociated with AT function and adipogenesis.
LB  - PUB:(DE-HGF)16
C6  - pmid:37766984
DO  - DOI:10.1016/j.isci.2023.107841
UR  - https://inrepo02.dkfz.de/record/283150
ER  -