Journal Article

DKFZ-2023-01937

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Spatiotemporal, optogenetic control of gene expression in organoids.

Legnini, I. ; Emmenegger, L. ; Zappulo, A. ; Rybak-Wolf, A. ; Wurmus, R. ; Martinez, A. O. ; Jara, C. C. ; Boltengagen, A. ; Hessler, T. ; Mastrobuoni, G. ; Kempa, S. ; Zinzen, R. ; Woehler, A. ; Rajewsky, N.DKFZ*

2023
Nature Publishing Group London [u.a.]

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Please use a persistent id in citations: doi:10.1038/s41592-023-01986-w

Abstract: Organoids derived from stem cells have become an increasingly important tool for studying human development and modeling disease. However, methods are still needed to control and study spatiotemporal patterns of gene expression in organoids. Here we combined optogenetics and gene perturbation technologies to activate or knock-down RNA of target genes in programmable spatiotemporal patterns. To illustrate the usefulness of our approach, we locally activated Sonic Hedgehog (SHH) signaling in an organoid model for human neurodevelopment. Spatial and single-cell transcriptomic analyses showed that this local induction was sufficient to generate stereotypically patterned organoids and revealed new insights into SHH's contribution to gene regulation in neurodevelopment. With this study, we propose optogenetic perturbations in combination with spatial transcriptomics as a powerful technology to reprogram and study cell fates and tissue patterning in organoids.

Note: 2023 Oct;20(10):1544-1552

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Contributing Institute(s):

1. DKTK Koordinierungsstelle Berlin (BE01)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2023

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Nature ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 40 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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