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000285437 041__ $$aEnglish
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000285437 1001_ $$aBoehm, Daniela$$b0
000285437 245__ $$aProtocol for an in vitro assay to study HIV-1 Tat methylation.
000285437 260__ $$aAmsterdam$$bElsevier$$c2023
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000285437 520__ $$aA critical virus-encoded regulator of HIV-1 transcription is the Tat protein, which is required to potently activate transcription. Tat is regulated by a wide variety of post-translational modifications. This protocol describes an in vitro assay to study Tat methylation. We describe steps for incorporation of radioactive methyl groups into Tat protein, visualization by gel analysis, Coomassie blue stain, gel drying, and detection by autoradiography. This protocol can also be used to assess methylation in other proteins such as histones. For complete details on the use and execution of this protocol, please refer to Boehm et al. (2023).1.
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000285437 650_7 $$2Other$$aCell Biology
000285437 650_7 $$2Other$$aMolecular Biology
000285437 650_7 $$2Other$$aProtein Biochemistry
000285437 7001_ $$aKaehlcke, Katrin$$b1
000285437 7001_ $$0P:(DE-HGF)0$$aSchnoelzer, Martina$$b2
000285437 7001_ $$aOtt, Melanie$$b3
000285437 773__ $$0PERI:(DE-600)3053335-1$$a10.1016/j.xpro.2023.102687$$gVol. 4, no. 4, p. 102687 -$$n4$$p102687$$tSTAR Protocols$$v4$$x2666-1667$$y2023
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