Journal Article

DKFZ-2023-02371

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protocol for an in vitro assay to study HIV-1 Tat methylation.

Boehm, D. ; Kaehlcke, K. ; Schnoelzer, M.DKFZ* ; Ott, M.

2023
Elsevier Amsterdam

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Please use a persistent id in citations: doi:10.1016/j.xpro.2023.102687

Abstract: A critical virus-encoded regulator of HIV-1 transcription is the Tat protein, which is required to potently activate transcription. Tat is regulated by a wide variety of post-translational modifications. This protocol describes an in vitro assay to study Tat methylation. We describe steps for incorporation of radioactive methyl groups into Tat protein, visualization by gel analysis, Coomassie blue stain, gel drying, and detection by autoradiography. This protocol can also be used to assess methylation in other proteins such as histones. For complete details on the use and execution of this protocol, please refer to Boehm et al. (2023).1.

Keyword(s): Cell Biology ; Molecular Biology ; Protein Biochemistry

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Contributing Institute(s):

1. Funktionelle Proteomanalyse (B100)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2023

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Database coverage:
; ; Article Processing Charges ; DOAJ Seal ; Fees ; PubMed Central ; SCOPUS

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