TY  - JOUR
AU  - Boehm, Daniela
AU  - Kaehlcke, Katrin
AU  - Schnoelzer, Martina
AU  - Ott, Melanie
TI  - Protocol for an in vitro assay to study HIV-1 Tat methylation.
JO  - STAR Protocols
VL  - 4
IS  - 4
SN  - 2666-1667
CY  - Amsterdam
PB  - Elsevier
M1  - DKFZ-2023-02371
SP  - 102687
PY  - 2023
AB  - A critical virus-encoded regulator of HIV-1 transcription is the Tat protein, which is required to potently activate transcription. Tat is regulated by a wide variety of post-translational modifications. This protocol describes an in vitro assay to study Tat methylation. We describe steps for incorporation of radioactive methyl groups into Tat protein, visualization by gel analysis, Coomassie blue stain, gel drying, and detection by autoradiography. This protocol can also be used to assess methylation in other proteins such as histones. For complete details on the use and execution of this protocol, please refer to Boehm et al. (2023).1.
KW  - Cell Biology (Other)
KW  - Molecular Biology (Other)
KW  - Protein Biochemistry (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37979180
DO  - DOI:10.1016/j.xpro.2023.102687
UR  - https://inrepo02.dkfz.de/record/285437
ER  -