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@ARTICLE{Boehm:285437,
      author       = {D. Boehm and K. Kaehlcke and M. Schnoelzer$^*$ and M. Ott},
      title        = {{P}rotocol for an in vitro assay to study {HIV}-1 {T}at
                      methylation.},
      journal      = {STAR Protocols},
      volume       = {4},
      number       = {4},
      issn         = {2666-1667},
      address      = {Amsterdam},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2023-02371},
      pages        = {102687},
      year         = {2023},
      abstract     = {A critical virus-encoded regulator of HIV-1 transcription
                      is the Tat protein, which is required to potently activate
                      transcription. Tat is regulated by a wide variety of
                      post-translational modifications. This protocol describes an
                      in vitro assay to study Tat methylation. We describe steps
                      for incorporation of radioactive methyl groups into Tat
                      protein, visualization by gel analysis, Coomassie blue
                      stain, gel drying, and detection by autoradiography. This
                      protocol can also be used to assess methylation in other
                      proteins such as histones. For complete details on the use
                      and execution of this protocol, please refer to Boehm et al.
                      (2023).1.},
      keywords     = {Cell Biology (Other) / Molecular Biology (Other) / Protein
                      Biochemistry (Other)},
      cin          = {B100},
      ddc          = {600},
      cid          = {I:(DE-He78)B100-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37979180},
      doi          = {10.1016/j.xpro.2023.102687},
      url          = {https://inrepo02.dkfz.de/record/285437},
}