TY  - JOUR
AU  - Paech, Daniel
AU  - Weckesser, Nina
AU  - Franke, Vanessa
AU  - Breitling, Johannes
AU  - Görke, Steffen
AU  - Deike-Hofmann, Katerina
AU  - Wick, Antje
AU  - Scherer, Moritz
AU  - Unterberg, Andreas
AU  - Wick, Wolfgang
AU  - Bendszus, Martin
AU  - Bachert, Peter
AU  - Ladd, Mark
AU  - Schlemmer, Heinz-Peter
AU  - Korzowski, Andreas
TI  - Whole-Brain Intracellular pH Mapping of Gliomas Using High-Resolution 31P MR Spectroscopic Imaging at 7.0 T.
JO  - Radiology / Imaging cancer
VL  - 6
IS  - 1
SN  - 2638-616X
CY  - Oak Brook, IL
PB  - RSNA, Radiological Society of North America
M1  - DKFZ-2023-02807
SP  - e220127
PY  - 2023
N1  - #EA:E010#LA:E020#
AB  - Malignant tumors commonly exhibit a reversed pH gradient compared with normal tissue, with a more acidic extracellular pH and an alkaline intracellular pH (pHi). In this prospective study, pHi values in gliomas were quantified using high-resolution phosphorous 31 (31P) spectroscopic MRI at 7.0 T and were used to correlate pHi alterations with histopathologic findings. A total of 12 participants (mean age, 58 years ± 18 [SD]; seven male, five female) with histopathologically proven, newly diagnosed glioma were included between September 2018 and November 2019. The 31P spectroscopic MRI scans were acquired using a double-resonant 31P/1H phased-array head coil together with a three-dimensional (3D) 31P chemical shift imaging sequence (5.7-mL voxel volume) performed with a 7.0-T whole-body system. The 3D volumetric segmentations were performed for the whole-tumor volumes (WTVs); tumor subcompartments of necrosis, gadolinium enhancement, and nonenhancing T2 (NCE T2) hyperintensity; and normal-appearing white matter (NAWM), and pHi values were compared. Spearman correlation was used to assess association between pHi and the proliferation index Ki-67. For all study participants, mean pHi values were higher in the WTV (7.057 ± 0.024) compared with NAWM (7.006 ± 0.012; P < .001). In eight participants with high-grade gliomas, pHi was increased in all tumor subcompartments (necrosis, 7.075 ± 0.033; gadolinium enhancement, 7.075 ± 0.024; NCE T2 hyperintensity, 7.043 ± 0.015) compared with NAWM (7.004 ± 0.014; all P < .01). The pHi values of WTV positively correlated with Ki-67 (R2 = 0.74, r = 0.78, P = .001). In conclusion, 31P spectroscopic MRI at 7.0 T enabled high-resolution quantification of pHi in gliomas, with pHi alteration associated with the Ki-67 proliferation index, and may aid in diagnosis and treatment monitoring. Keywords: 31P MRSI, pH, Glioma, Glioblastoma, Ultra-High-Field MRI, Imaging Biomarker, 7 Tesla Supplemental material is available for this article. © RSNA, 2023.
KW  - Male
KW  - Humans
KW  - Female
KW  - Middle Aged
KW  - Contrast Media
KW  - Prospective Studies
KW  - Gadolinium
KW  - Ki-67 Antigen
KW  - Brain Neoplasms: diagnostic imaging
KW  - Glioma: diagnostic imaging
KW  - Magnetic Resonance Imaging: methods
KW  - Brain: pathology
KW  - Necrosis
KW  - Hydrogen-Ion Concentration
KW  - 31P MRSI (Other)
KW  - 7 Tesla (Other)
KW  - Glioblastoma (Other)
KW  - Glioma (Other)
KW  - Imaging Biomarker (Other)
KW  - Ultra-High-Field MRI (Other)
KW  - pH (Other)
KW  - Contrast Media (NLM Chemicals)
KW  - Gadolinium (NLM Chemicals)
KW  - Ki-67 Antigen (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:38133553
DO  - DOI:10.1148/rycan.220127
UR  - https://inrepo02.dkfz.de/record/286590
ER  -