Journal Article

DKFZ-2024-00894

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Human ABC and SLC Transporters: The Culprit Responsible for Unspecific PSMA-617 Uptake?

Tas, H. (First author)DKFZ* ; Bakos, G.DKFZ* ; Bauder-Wüst, U.DKFZ* ; Schäfer, M.DKFZ* ; Remde, Y.DKFZ* ; Roscher, M.DKFZ* ; Benesova-Schäfer, M. (Last author)DKFZ*

2024
MDPI Basel

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Please use a persistent id in citations: doi:10.3390/ph17040513

Abstract: [177Lu]Lu-PSMA-617 has recently been successfully approved by the FDA, the MHRA, Health Canada and the EMA as Pluvicto®. However, salivary gland (SG) and kidney toxicities account for its main dose-limiting side-effects, while its corresponding uptake and retention mechanisms still remain elusive. Recently, the presence of different ATP-binding cassette (ABC) transporters, such as human breast cancer resistance proteins (BCRP), multidrug resistance proteins (MDR1), multidrug-resistance-related proteins (MRP1, MRP4) and solute cassette (SLC) transporters, such as multidrug and toxin extrusion proteins (MATE1, MATE2-K), organic anion transporters (OAT1, OAT2v1, OAT3, OAT4) and peptide transporters (PEPT2), has been verified at different abundances in human SGs and kidneys. Therefore, our aim was to assess whether [177Lu]Lu-PSMA-617 and [225Ac]Ac-PSMA-617 are substrates of these ABC and SLC transporters. For in vitro studies, the novel isotopologue ([α,β-3H]Nal)Lu-PSMA-617 was used in cell lines or vesicles expressing the aforementioned human ABC and SLC transporters for inhibition and uptake studies, respectively. The corresponding probe substrates and reference inhibitors were used as controls. Our results indicate that [177Lu]Lu-PSMA-617 and [225Ac]Ac-PSMA-617 are neither inhibitors nor substrates of the examined transporters. Therefore, our results show that human ABC and SLC transporters play no central role in the uptake and retention of [177Lu]Lu-PSMA-617 and [225Ac]Ac-PSMA-617 in the SGs and kidneys nor in the observed toxicities.

Keyword(s): PSMA ; PSMA-617 ; efflux transporters ; kidney toxicity ; prostate cancer ; salivary gland toxicity ; side-effects ; targeted alpha therapy ; targeted radionuclide therapy ; uptake transporters

Note: #EA:E270#LA:E270#

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Contributing Institute(s):

1. Translationale Radiotheranostik (E270)
2. Radiopharmazeutika und Präklinische Studien (W630)

Research Program(s):

1. 315 - Bildgebung und Radioonkologie (POF4-315) (POF4-315)

Appears in the scientific report 2024

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