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@ARTICLE{Aslam:290204,
author = {A. Aslam and F. Masood and K. Perveen and M. Berger$^*$ and
A. Pervaiz and M. Zepp$^*$ and K. D. Klika$^*$ and T. Yasin
and A. Hameed},
title = {{P}reparation, characterization and evaluation of
{HP}β{CD}-{PTX}/{PHB} nanoparticles for p{H}-responsive,
cytotoxic and apoptotic properties.},
journal = {International journal of biological macromolecules},
volume = {270},
number = {Part 2},
issn = {0141-8130},
address = {New York, NY [u.a.]},
publisher = {Elsevier},
reportid = {DKFZ-2024-01037},
pages = {132268},
year = {2024},
note = {Volume 270, Part 2, June 2024, 132268},
abstract = {Paclitaxel (PTX) is a potent anticancer drug. However, PTX
exhibits extremely poor solubility in aqueous solution along
with severe side effects. Therefore, in this study, an
inclusion complex was prepared between PTX and
hydroxypropyl-β-cyclodextrin (HPβCD) by solvent
evaporation to enhance the drug's solubility. The HPβCD-PTX
inclusion complex was then encapsulated in
poly-3-hydroxybutyrate (PHB) to fabricate drug-loaded
nanoparticles (HPβCD-PTX/PHB NPs) by nanoprecipitation. The
HPβCD-PTX/PHB NPs depicted a higher release of PTX at pH
5.5 thus demonstrating a pH-dependent release profile. The
cytotoxic properties of HPβCD-PTX/PHB NPs were tested
against MCF-7, MDA-MB-231 and SW-620 cell lines. The
cytotoxic potential of HPβCD-PTX/PHB NPs was 2.59-fold
improved in MCF-7 cells in comparison to free PTX.
Additionally, the HPβCD-PTX/PHB NPs improved the
antimitotic (1.68-fold) and apoptotic (8.45-fold) effects of
PTX in MCF-7 cells in comparison to PTX alone. In summary,
these pH-responsive nanoparticles could be prospective
carriers for enhancing the cytotoxic properties of PTX for
the treatment of breast cancer.},
keywords = {HPβCD-drug inclusion complex (Other) / Nano-system (Other)
/ PHB (Other) / Paclitaxel (Other)},
cin = {G401 / A390},
ddc = {570},
cid = {I:(DE-He78)G401-20160331 / I:(DE-He78)A390-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38734336},
doi = {10.1016/j.ijbiomac.2024.132268},
url = {https://inrepo02.dkfz.de/record/290204},
}