TY  - JOUR
AU  - Rosenkranz, Micha
AU  - Nkumama, Irene Nailain
AU  - Ogwang, Rodney
AU  - Kraker, Sara
AU  - Blickling, Marie
AU  - Mwai, Kennedy
AU  - Odera, Dennis
AU  - Tuju, James
AU  - Fürle, Kristin
AU  - Frank, Roland
AU  - Chepsat, Emily
AU  - Kapulu, Melissa C
AU  - Study Team, Chmi-Sika
AU  - Osier, Faith Ha
TI  - Full-length MSP1 is a major target of protective immunity after controlled human malaria infection.
JO  - Life science alliance
VL  - 7
IS  - 8
SN  - 2575-1077
CY  - Heidelberg
PB  - EMBO Press
M1  - DKFZ-2024-01139
SP  - e202301910 -
PY  - 2024
N1  - B Cell Immunology, German Cancer Research Center
AB  - The merozoite surface protein 1 (MSP1) is the most abundant protein on the surface of the invasive merozoite stages of Plasmodium falciparum and has long been considered a key target of protective immunity. We used samples from a single controlled human malaria challenge study to test whether the full-length version of MSP1 (MSP1FL) induced antibodies that mediated Fc-IgG functional activity in five independent assays. We found that anti-MSP1FL antibodies induced complement fixation via C1q, monocyte-mediated phagocytosis, neutrophil respiratory burst, and natural killer cell degranulation as well as IFNγ production. Activity in each of these assays was strongly associated with protection. The breadth of MSP1-specific Fc-mediated effector functions was more strongly associated with protection than the individual measures and closely mirrored what we have previously reported using the same assays against merozoites. Our findings suggest that MSP1FL is an important target of functional antibodies that contribute to a protective immune response against malaria.
KW  - Humans
KW  - Merozoite Surface Protein 1: immunology
KW  - Malaria, Falciparum: immunology
KW  - Malaria, Falciparum: parasitology
KW  - Plasmodium falciparum: immunology
KW  - Antibodies, Protozoan: immunology
KW  - Phagocytosis: immunology
KW  - Immunoglobulin G: immunology
KW  - Killer Cells, Natural: immunology
KW  - Killer Cells, Natural: metabolism
KW  - Interferon-gamma: metabolism
KW  - Interferon-gamma: immunology
KW  - Female
KW  - Merozoites: immunology
KW  - Neutrophils: immunology
KW  - Neutrophils: metabolism
KW  - Merozoite Surface Protein 1 (NLM Chemicals)
KW  - Antibodies, Protozoan (NLM Chemicals)
KW  - Immunoglobulin G (NLM Chemicals)
KW  - Interferon-gamma (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:38803222
DO  - DOI:10.26508/lsa.202301910
UR  - https://inrepo02.dkfz.de/record/290512
ER  -