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@ARTICLE{Hirth:291567,
author = {A. Hirth$^*$ and E. Fatti$^*$ and E. Netz and S. P.
Acebron$^*$ and D. Papageorgiou$^*$ and A. Svorinic$^*$ and
C.-M. Cruciat$^*$ and E. Karaulanov and A. Gopanenko and T.
Zhu$^*$ and I. Sinning and J. Krijgsveld$^*$ and O.
Kohlbacher and C. Niehrs$^*$},
title = {{DEAD} box {RNA} helicases are pervasive protein kinase
interactors and activators.},
journal = {Genome research},
volume = {34},
number = {6},
issn = {1054-9803},
address = {Cold Spring Harbor, NY},
publisher = {Laboratory Press},
reportid = {DKFZ-2024-01452},
pages = {952-966},
year = {2024},
note = {DKFZ-ZMBH Alliance / #EA:A050#LA:A050# / 2024 Jul
23;34(6):952-966},
abstract = {DEAD box (DDX) RNA helicases are a large family of ATPases,
many of which have unknown functions. There is emerging
evidence that besides their role in RNA biology, DDX
proteins may stimulate protein kinases. To investigate if
protein kinase-DDX interaction is a more widespread
phenomenon, we conducted three orthogonal large-scale
screens, including proteomics analysis with 32 RNA
helicases, protein array profiling, and kinome-wide in vitro
kinase assays. We retrieved Ser/Thr protein kinases as
prominent interactors of RNA helicases and report hundreds
of binary interactions. We identified members of ten protein
kinase families, which bind to, and are stimulated by, DDX
proteins, including CDK, CK1, CK2, DYRK, MARK, NEK, PRKC,
SRPK, STE7/MAP2K, and STE20/PAK family members. We
identified MARK1 in all screens and validated that DDX
proteins accelerate the MARK1 catalytic rate. These findings
indicate pervasive interactions between protein kinases and
DEAD box RNA helicases, and provide a rich resource to
explore their regulatory relationships.},
cin = {B230 / A050},
ddc = {540},
cid = {I:(DE-He78)B230-20160331 / I:(DE-He78)A050-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38986579},
doi = {10.1101/gr.278264.123},
url = {https://inrepo02.dkfz.de/record/291567},
}