Journal Article

DKFZ-2024-01452

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png DEAD box RNA helicases are pervasive protein kinase interactors and activators.

Hirth, A. (First author)DKFZ* ; Fatti, E. (First author)DKFZ* ; Netz, E. ; Acebron, S. P.DKFZ* ; Papageorgiou, D.DKFZ* ; Svorinic, A.DKFZ* ; Cruciat, C.-M.DKFZ* ; Karaulanov, E. ; Gopanenko, A. ; Zhu, T.DKFZ* ; Sinning, I. ; Krijgsveld, J.DKFZ* ; Kohlbacher, O. ; Niehrs, C. (Last author)DKFZ*

2024
Laboratory Press Cold Spring Harbor, NY

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Please use a persistent id in citations: doi:10.1101/gr.278264.123

Abstract: DEAD box (DDX) RNA helicases are a large family of ATPases, many of which have unknown functions. There is emerging evidence that besides their role in RNA biology, DDX proteins may stimulate protein kinases. To investigate if protein kinase-DDX interaction is a more widespread phenomenon, we conducted three orthogonal large-scale screens, including proteomics analysis with 32 RNA helicases, protein array profiling, and kinome-wide in vitro kinase assays. We retrieved Ser/Thr protein kinases as prominent interactors of RNA helicases and report hundreds of binary interactions. We identified members of ten protein kinase families, which bind to, and are stimulated by, DDX proteins, including CDK, CK1, CK2, DYRK, MARK, NEK, PRKC, SRPK, STE7/MAP2K, and STE20/PAK family members. We identified MARK1 in all screens and validated that DDX proteins accelerate the MARK1 catalytic rate. These findings indicate pervasive interactions between protein kinases and DEAD box RNA helicases, and provide a rich resource to explore their regulatory relationships.

Note: DKFZ-ZMBH Alliance / #EA:A050#LA:A050# / 2024 Jul 23;34(6):952-966

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Contributing Institute(s):

1. B230 Proteomik von Stammzellen und Krebs (B230)
2. A050 Molekulare Embryologie (A050)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2024

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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