%0 Journal Article
%A Parasyraki, Eleftheria
%A Mallick, Medhavi
%A Hatch, Victoria
%A Vastolo, Viviana
%A Musheev, Michael U
%A Karaulanov, Emil
%A Gopanenko, Alexandr
%A Moxon, Simon
%A Méndez-Lago, Maria
%A Han, Dandan
%A Schomacher, Lars
%A Mukherjee, Debasish
%A Niehrs, Christof
%T 5-Formylcytosine is an activating epigenetic mark for RNA Pol III during zygotic reprogramming.
%J Cell
%V 187
%N 21
%@ 0092-8674
%C New York, NY
%I Elsevier
%M DKFZ-2024-01772
%P 6088-6103.e18
%D 2024
%Z #LA:A050# / 2024 Oct 17;187(21):6088-6103.e18 / DKFZ-ZMBH Alliance
%X 5-Methylcytosine (5mC) is an established epigenetic mark in vertebrate genomic DNA, but whether its oxidation intermediates formed during TET-mediated DNA demethylation possess an instructive role of their own that is also physiologically relevant remains unresolved. Here, we reveal a 5-formylcytosine (5fC) nuclear chromocenter, which transiently forms during zygotic genome activation (ZGA) in Xenopus and mouse embryos. We identify this chromocenter as the perinucleolar compartment, a structure associated with RNA Pol III transcription. In Xenopus embryos, 5fC is highly enriched on Pol III target genes activated at ZGA, notably at oocyte-type tandem arrayed tRNA genes. By manipulating Tet and Tdg enzymes, we show that 5fC is required as a regulatory mark to promote Pol III recruitment as well as tRNA expression. Concordantly, 5fC modification of a tRNA transgene enhances its expression in vivo. The results establish 5fC as an activating epigenetic mark during zygotic reprogramming of Pol III gene expression.
%K 5-formylcytosine (Other)
%K B-box (Other)
%K RNA Pol III (Other)
%K TDG (Other)
%K TET (Other)
%K Xenopus (Other)
%K ZGA (Other)
%K perinucleolar compartment (Other)
%K tRNA (Other)
%K tRNA-iMet (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:39214079
%R 10.1016/j.cell.2024.08.011
%U https://inrepo02.dkfz.de/record/292526