Journal Article

DKFZ-2024-01772

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png 5-Formylcytosine is an activating epigenetic mark for RNA Pol III during zygotic reprogramming.

Parasyraki, E. ; Mallick, M. ; Hatch, V. ; Vastolo, V. ; Musheev, M. U. ; Karaulanov, E. ; Gopanenko, A. ; Moxon, S. ; Méndez-Lago, M. ; Han, D. ; Schomacher, L. ; Mukherjee, D. ; Niehrs, C. (Last author)DKFZ*

2024
Elsevier New York, NY

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Please use a persistent id in citations: doi:10.1016/j.cell.2024.08.011

Abstract: 5-Methylcytosine (5mC) is an established epigenetic mark in vertebrate genomic DNA, but whether its oxidation intermediates formed during TET-mediated DNA demethylation possess an instructive role of their own that is also physiologically relevant remains unresolved. Here, we reveal a 5-formylcytosine (5fC) nuclear chromocenter, which transiently forms during zygotic genome activation (ZGA) in Xenopus and mouse embryos. We identify this chromocenter as the perinucleolar compartment, a structure associated with RNA Pol III transcription. In Xenopus embryos, 5fC is highly enriched on Pol III target genes activated at ZGA, notably at oocyte-type tandem arrayed tRNA genes. By manipulating Tet and Tdg enzymes, we show that 5fC is required as a regulatory mark to promote Pol III recruitment as well as tRNA expression. Concordantly, 5fC modification of a tRNA transgene enhances its expression in vivo. The results establish 5fC as an activating epigenetic mark during zygotic reprogramming of Pol III gene expression.

Keyword(s): 5-formylcytosine ; B-box ; RNA Pol III ; TDG ; TET ; Xenopus ; ZGA ; perinucleolar compartment ; tRNA ; tRNA-iMet

Note: #LA:A050# / 2024 Oct 17;187(21):6088-6103.e18 / DKFZ-ZMBH Alliance

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Contributing Institute(s):

1. A050 Molekulare Embryologie (A050)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2024

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 60 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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