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@ARTICLE{Parasyraki:292526,
author = {E. Parasyraki and M. Mallick and V. Hatch and V. Vastolo
and M. U. Musheev and E. Karaulanov and A. Gopanenko and S.
Moxon and M. Méndez-Lago and D. Han and L. Schomacher and
D. Mukherjee and C. Niehrs$^*$},
title = {5-{F}ormylcytosine is an activating epigenetic mark for
{RNA} {P}ol {III} during zygotic reprogramming.},
journal = {Cell},
volume = {187},
number = {21},
issn = {0092-8674},
address = {New York, NY},
publisher = {Elsevier},
reportid = {DKFZ-2024-01772},
pages = {6088-6103.e18},
year = {2024},
note = {#LA:A050# / 2024 Oct 17;187(21):6088-6103.e18 / DKFZ-ZMBH
Alliance},
abstract = {5-Methylcytosine (5mC) is an established epigenetic mark in
vertebrate genomic DNA, but whether its oxidation
intermediates formed during TET-mediated DNA demethylation
possess an instructive role of their own that is also
physiologically relevant remains unresolved. Here, we reveal
a 5-formylcytosine (5fC) nuclear chromocenter, which
transiently forms during zygotic genome activation (ZGA) in
Xenopus and mouse embryos. We identify this chromocenter as
the perinucleolar compartment, a structure associated with
RNA Pol III transcription. In Xenopus embryos, 5fC is highly
enriched on Pol III target genes activated at ZGA, notably
at oocyte-type tandem arrayed tRNA genes. By manipulating
Tet and Tdg enzymes, we show that 5fC is required as a
regulatory mark to promote Pol III recruitment as well as
tRNA expression. Concordantly, 5fC modification of a tRNA
transgene enhances its expression in vivo. The results
establish 5fC as an activating epigenetic mark during
zygotic reprogramming of Pol III gene expression.},
keywords = {5-formylcytosine (Other) / B-box (Other) / RNA Pol III
(Other) / TDG (Other) / TET (Other) / Xenopus (Other) / ZGA
(Other) / perinucleolar compartment (Other) / tRNA (Other) /
tRNA-iMet (Other)},
cin = {A050},
ddc = {610},
cid = {I:(DE-He78)A050-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39214079},
doi = {10.1016/j.cell.2024.08.011},
url = {https://inrepo02.dkfz.de/record/292526},
}