Home > Publications database > 5-Formylcytosine is an activating epigenetic mark for RNA Pol III during zygotic reprogramming. > print

001     292526
005     20250819103400.0
024 7 _ |a 10.1016/j.cell.2024.08.011
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100 1 _ |a Parasyraki, Eleftheria
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245 _ _ |a 5-Formylcytosine is an activating epigenetic mark for RNA Pol III during zygotic reprogramming.
260 _ _ |a New York, NY
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520 _ _ |a 5-Methylcytosine (5mC) is an established epigenetic mark in vertebrate genomic DNA, but whether its oxidation intermediates formed during TET-mediated DNA demethylation possess an instructive role of their own that is also physiologically relevant remains unresolved. Here, we reveal a 5-formylcytosine (5fC) nuclear chromocenter, which transiently forms during zygotic genome activation (ZGA) in Xenopus and mouse embryos. We identify this chromocenter as the perinucleolar compartment, a structure associated with RNA Pol III transcription. In Xenopus embryos, 5fC is highly enriched on Pol III target genes activated at ZGA, notably at oocyte-type tandem arrayed tRNA genes. By manipulating Tet and Tdg enzymes, we show that 5fC is required as a regulatory mark to promote Pol III recruitment as well as tRNA expression. Concordantly, 5fC modification of a tRNA transgene enhances its expression in vivo. The results establish 5fC as an activating epigenetic mark during zygotic reprogramming of Pol III gene expression.
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650 _ 7 |a 5-formylcytosine
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650 _ 7 |a B-box
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650 _ 7 |a RNA Pol III
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650 _ 7 |a TDG
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650 _ 7 |a TET
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650 _ 7 |a Xenopus
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650 _ 7 |a ZGA
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650 _ 7 |a perinucleolar compartment
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650 _ 7 |a tRNA
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650 _ 7 |a tRNA-iMet
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700 1 _ |a Mallick, Medhavi
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700 1 _ |a Hatch, Victoria
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700 1 _ |a Vastolo, Viviana
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700 1 _ |a Musheev, Michael U
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700 1 _ |a Karaulanov, Emil
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700 1 _ |a Gopanenko, Alexandr
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700 1 _ |a Moxon, Simon
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700 1 _ |a Méndez-Lago, Maria
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700 1 _ |a Han, Dandan
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700 1 _ |a Schomacher, Lars
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700 1 _ |a Mukherjee, Debasish
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700 1 _ |a Niehrs, Christof
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773 _ _ |a 10.1016/j.cell.2024.08.011
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856 4 _ |u https://inrepo02.dkfz.de/record/292526/files/1-s2.0-S0092867424009024-main.pdf
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