TY  - JOUR
AU  - Genard, Geraldine
AU  - Tirinato, Luca
AU  - Pagliari, Francesca
AU  - Da Silva, Jessica
AU  - Giammona, Alessandro
AU  - Alquraish, Fatema
AU  - Reyes, Maria Parra
AU  - Bordas, Marie
AU  - Marafioti, Maria Grazia
AU  - Franco, Simone Di
AU  - Janssen, Jeannette
AU  - Garcia-Calderón, Daniel
AU  - Hanley, Rachel
AU  - Nistico, Clelia
AU  - Fukasawa, Yoshinori
AU  - Müller, Torsten
AU  - Krijgsveld, Jeroen
AU  - Todaro, Matilde
AU  - Costanzo, Francesco Saverio
AU  - Stassi, Giorgio
AU  - Nessling, Michelle
AU  - Richter, Karsten
AU  - Maass, Kendra K
AU  - Liberale, Carlo
AU  - Seco, Joao
TI  - Lipid droplets and small extracellular vesicles: More than two independent entities.
JO  - Journal of extracellular biology
VL  - 3
IS  - 9
SN  - 2768-2811
CY  - Hoboken, New Jersey
PB  - Wiley
M1  - DKFZ-2024-01847
SP  - e162
PY  - 2024
N1  - #EA:E041#LA:E041#
AB  - Despite increasing knowledge about small extracellular vesicle (sEV) composition and functions in cell-cell communication, the mechanism behind their biogenesis remains unclear. Here, we reveal for the first time that sEV biogenesis and release into the microenvironment are tightly connected with another important organelle, Lipid Droplets (LDs). The correlation was observed in several human cancer cell lines as well as patient-derived colorectal cancer stem cells (CR-CSCs). Our results demonstrated that external stimuli such as radiation, pH, hypoxia or lipid-interfering drugs, known to affect the number of LDs/cell, similarly influenced sEV secretion. Importantly, through multiple omics data, at both mRNA and protein levels, we revealed RAB5C as a potential important molecular player behind this organelle connection. Altogether, the potential to fine-tune sEV biogenesis by targeting LDs could significantly impact the amount, cargos and properties of these sEVs, opening new clinical perspectives.
KW  - Rab (Other)
KW  - exosomes (Other)
KW  - hypoxia (Other)
KW  - iron metabolism (Other)
KW  - irradiation (Other)
KW  - lipid droplets (Other)
KW  - pH (Other)
KW  - small extracellular vesicles (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:39257626
C2  - pmc:PMC11386333
DO  - DOI:10.1002/jex2.162
UR  - https://inrepo02.dkfz.de/record/293274
ER  -