%0 Journal Article
%A Kuenzel, Natascha Andrea
%A Dobner, Jochen
%A Reichert, Doreen
%A Rossi, Andrea
%A Boukamp, Petra
%A Esser, Charlotte
%T Vδ1 T cells integrated in full thickness skin equivalents: a model for the role of human skin-resident γδT cells.
%J The journal of investigative dermatology
%V 145
%N 6
%@ 0022-202X
%C Amsterdam
%I Elsevier
%M DKFZ-2024-02032
%P 1407-1421
%D 2025
%Z #LA:A110# / Volume 145, Issue 6, June 2025, Pages 1407-1421 / ZZ01
%X Vδ1 T cells are a subpopulation of γδT cells found in human dermis. In contrast to murine skin-resident γδT cells, much less is known regarding their role and function in skin health and disease. Here we report the successful integration of Vδ1 T cells into long-term fibroblast-derived matrix skin equivalents (SE). We isolated Vδ1 T cells from human blood, where they are rare, and established conditions for the integration and maintenance of the freshly isolated Vδ1 T cells in the SEs. Plated on top of the dermal equivalents (DEs), almost all Vδ1 T cells migrated into the dermal matrix where they exerted their influence on both the DE and the epithelium. Vδ1 T cells contributed to epidermal differentiation as indicated by histology, expression of epidermal differentiation markers and RNAseq expression profile. When complemented with the carcinoma-derived SCC13 cells instead of HaCaT, our data suggest a role for Vδ1 T cells in slowing growth of the tumor cells, as indicated by reduced stratification and changes in gene expression profiles. Together, we demonstrate the successful establishment of human Vδ1 T cell-competent skin and skin carcinoma equivalents (SE, SCE) and provide evidence for molecular and functional consequences of the Vδ1 T cells on their respective environment.
%K Vδ1 T cells (Other)
%K human immunocompetent skin model (Other)
%K immune surveillance (Other)
%K squamous cell carcinoma (Other)
%K γδT cells (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:39384018
%R 10.1016/j.jid.2024.08.037
%U https://inrepo02.dkfz.de/record/293972