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| Home > Publications database > Vδ1 T cells integrated in full thickness skin equivalents: a model for the role of human skin-resident γδT cells. |
| Home > Publications database > Vδ1 T cells integrated in full thickness skin equivalents: a model for the role of human skin-resident γδT cells. |
| Journal Article | DKFZ-2024-02032 |
; ; ; ; ;
2025
Elsevier
Amsterdam
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Please use a persistent id in citations: doi:10.1016/j.jid.2024.08.037
Abstract: Vδ1 T cells are a subpopulation of γδT cells found in human dermis. In contrast to murine skin-resident γδT cells, much less is known regarding their role and function in skin health and disease. Here we report the successful integration of Vδ1 T cells into long-term fibroblast-derived matrix skin equivalents (SE). We isolated Vδ1 T cells from human blood, where they are rare, and established conditions for the integration and maintenance of the freshly isolated Vδ1 T cells in the SEs. Plated on top of the dermal equivalents (DEs), almost all Vδ1 T cells migrated into the dermal matrix where they exerted their influence on both the DE and the epithelium. Vδ1 T cells contributed to epidermal differentiation as indicated by histology, expression of epidermal differentiation markers and RNAseq expression profile. When complemented with the carcinoma-derived SCC13 cells instead of HaCaT, our data suggest a role for Vδ1 T cells in slowing growth of the tumor cells, as indicated by reduced stratification and changes in gene expression profiles. Together, we demonstrate the successful establishment of human Vδ1 T cell-competent skin and skin carcinoma equivalents (SE, SCE) and provide evidence for molecular and functional consequences of the Vδ1 T cells on their respective environment.
Keyword(s): Vδ1 T cells ; human immunocompetent skin model ; immune surveillance ; squamous cell carcinoma ; γδT cells
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