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000293972 1001_ $$aKuenzel, Natascha Andrea$$b0
000293972 245__ $$aVδ1 T cells integrated in full thickness skin equivalents: a model for the role of human skin-resident γδT cells.
000293972 260__ $$aAmsterdam$$bElsevier$$c2025
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000293972 500__ $$a#LA:A110# / Volume 145, Issue 6, June 2025, Pages 1407-1421 / ZZ01
000293972 520__ $$aVδ1 T cells are a subpopulation of γδT cells found in human dermis. In contrast to murine skin-resident γδT cells, much less is known regarding their role and function in skin health and disease. Here we report the successful integration of Vδ1 T cells into long-term fibroblast-derived matrix skin equivalents (SE). We isolated Vδ1 T cells from human blood, where they are rare, and established conditions for the integration and maintenance of the freshly isolated Vδ1 T cells in the SEs. Plated on top of the dermal equivalents (DEs), almost all Vδ1 T cells migrated into the dermal matrix where they exerted their influence on both the DE and the epithelium. Vδ1 T cells contributed to epidermal differentiation as indicated by histology, expression of epidermal differentiation markers and RNAseq expression profile. When complemented with the carcinoma-derived SCC13 cells instead of HaCaT, our data suggest a role for Vδ1 T cells in slowing growth of the tumor cells, as indicated by reduced stratification and changes in gene expression profiles. Together, we demonstrate the successful establishment of human Vδ1 T cell-competent skin and skin carcinoma equivalents (SE, SCE) and provide evidence for molecular and functional consequences of the Vδ1 T cells on their respective environment.
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000293972 650_7 $$2Other$$aVδ1 T cells
000293972 650_7 $$2Other$$ahuman immunocompetent skin model
000293972 650_7 $$2Other$$aimmune surveillance
000293972 650_7 $$2Other$$asquamous cell carcinoma
000293972 650_7 $$2Other$$aγδT cells
000293972 7001_ $$aDobner, Jochen$$b1
000293972 7001_ $$aReichert, Doreen$$b2
000293972 7001_ $$aRossi, Andrea$$b3
000293972 7001_ $$0P:(DE-He78)c1895aa471c7ac9c7173045464b69b31$$aBoukamp, Petra$$b4$$eLast author$$udkfz
000293972 7001_ $$aEsser, Charlotte$$b5
000293972 773__ $$0PERI:(DE-600)2006902-9$$a10.1016/j.jid.2024.08.037$$gp. S0022202X24021730$$n6$$p1407-1421$$tThe journal of investigative dermatology$$v145$$x0022-202X$$y2025
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