TY  - JOUR
AU  - Kuenzel, Natascha Andrea
AU  - Dobner, Jochen
AU  - Reichert, Doreen
AU  - Rossi, Andrea
AU  - Boukamp, Petra
AU  - Esser, Charlotte
TI  - Vδ1 T cells integrated in full thickness skin equivalents: a model for the role of human skin-resident γδT cells.
JO  - The journal of investigative dermatology
VL  - 145
IS  - 6
SN  - 0022-202X
CY  - Amsterdam
PB  - Elsevier
M1  - DKFZ-2024-02032
SP  - 1407-1421
PY  - 2025
N1  - #LA:A110# / Volume 145, Issue 6, June 2025, Pages 1407-1421 / ZZ01
AB  - Vδ1 T cells are a subpopulation of γδT cells found in human dermis. In contrast to murine skin-resident γδT cells, much less is known regarding their role and function in skin health and disease. Here we report the successful integration of Vδ1 T cells into long-term fibroblast-derived matrix skin equivalents (SE). We isolated Vδ1 T cells from human blood, where they are rare, and established conditions for the integration and maintenance of the freshly isolated Vδ1 T cells in the SEs. Plated on top of the dermal equivalents (DEs), almost all Vδ1 T cells migrated into the dermal matrix where they exerted their influence on both the DE and the epithelium. Vδ1 T cells contributed to epidermal differentiation as indicated by histology, expression of epidermal differentiation markers and RNAseq expression profile. When complemented with the carcinoma-derived SCC13 cells instead of HaCaT, our data suggest a role for Vδ1 T cells in slowing growth of the tumor cells, as indicated by reduced stratification and changes in gene expression profiles. Together, we demonstrate the successful establishment of human Vδ1 T cell-competent skin and skin carcinoma equivalents (SE, SCE) and provide evidence for molecular and functional consequences of the Vδ1 T cells on their respective environment.
KW  - Vδ1 T cells (Other)
KW  - human immunocompetent skin model (Other)
KW  - immune surveillance (Other)
KW  - squamous cell carcinoma (Other)
KW  - γδT cells (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:39384018
DO  - DOI:10.1016/j.jid.2024.08.037
UR  - https://inrepo02.dkfz.de/record/293972
ER  -