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@ARTICLE{Kuenzel:293972,
      author       = {N. A. Kuenzel and J. Dobner and D. Reichert and A. Rossi
                      and P. Boukamp$^*$ and C. Esser},
      title        = {{V}δ1 {T} cells integrated in full thickness skin
                      equivalents: a model for the role of human skin-resident
                      γδ{T} cells.},
      journal      = {The journal of investigative dermatology},
      volume       = {145},
      number       = {6},
      issn         = {0022-202X},
      address      = {Amsterdam},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2024-02032},
      pages        = {1407-1421},
      year         = {2025},
      note         = {#LA:A110# / Volume 145, Issue 6, June 2025, Pages 1407-1421
                      / ZZ01},
      abstract     = {Vδ1 T cells are a subpopulation of γδT cells found in
                      human dermis. In contrast to murine skin-resident γδT
                      cells, much less is known regarding their role and function
                      in skin health and disease. Here we report the successful
                      integration of Vδ1 T cells into long-term
                      fibroblast-derived matrix skin equivalents (SE). We isolated
                      Vδ1 T cells from human blood, where they are rare, and
                      established conditions for the integration and maintenance
                      of the freshly isolated Vδ1 T cells in the SEs. Plated on
                      top of the dermal equivalents (DEs), almost all Vδ1 T cells
                      migrated into the dermal matrix where they exerted their
                      influence on both the DE and the epithelium. Vδ1 T cells
                      contributed to epidermal differentiation as indicated by
                      histology, expression of epidermal differentiation markers
                      and RNAseq expression profile. When complemented with the
                      carcinoma-derived SCC13 cells instead of HaCaT, our data
                      suggest a role for Vδ1 T cells in slowing growth of the
                      tumor cells, as indicated by reduced stratification and
                      changes in gene expression profiles. Together, we
                      demonstrate the successful establishment of human Vδ1 T
                      cell-competent skin and skin carcinoma equivalents (SE, SCE)
                      and provide evidence for molecular and functional
                      consequences of the Vδ1 T cells on their respective
                      environment.},
      keywords     = {Vδ1 T cells (Other) / human immunocompetent skin model
                      (Other) / immune surveillance (Other) / squamous cell
                      carcinoma (Other) / γδT cells (Other)},
      cin          = {A110},
      ddc          = {610},
      cid          = {I:(DE-He78)A110-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39384018},
      doi          = {10.1016/j.jid.2024.08.037},
      url          = {https://inrepo02.dkfz.de/record/293972},
}