TY  - JOUR
AU  - Vidal-Vázquez, Nicolás
AU  - Hernández-Núñez, Ismael
AU  - Carballo-Pacoret, Pablo
AU  - Salisbury, Sarah
AU  - Villamayor, Paula R
AU  - Hervas-Sotomayor, Francisca
AU  - Yuan, Xuefei
AU  - Lamanna, Francesco
AU  - Schneider, Céline
AU  - Schmidt, Julia
AU  - Mazan, Sylvie
AU  - Kaessmann, Henrik
AU  - Adrio, Fátima
AU  - Robledo, Diego
AU  - Barreiro-Iglesias, Antón
AU  - Candal, Eva
TI  - A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula.
JO  - Scientific data
VL  - 12
IS  - 1
SN  - 2052-4436
CY  - London
PB  - Nature Publ. Group
M1  - DKFZ-2025-00313
SP  - 228
PY  - 2025
N1  - DKFZ-ZMBH Alliance
AB  - The retina, whose basic cellular structure is highly conserved across vertebrates, constitutes an accessible system for studying the central nervous system. In recent years, single-cell RNA sequencing studies have uncovered cellular diversity in the retina of a variety of species, providing new insights on retinal evolution and development. However, similar data in cartilaginous fishes, the sister group to all other extant jawed vertebrates, are still lacking. Here, we present a single-nucleus RNA sequencing atlas of the postnatal retina of the catshark Scyliorhinus canicula, consisting of the expression profiles for 17,438 individual cells from three female, juvenile catshark specimens. Unsupervised clustering revealed 22 distinct cell types comprising all major retinal cell classes, as well as retinal progenitor cells (whose presence reflects the persistence of proliferative activity in postnatal stages in sharks) and oligodendrocytes. Thus, our dataset serves as a foundation for further studies on the development and function of the catshark retina. Moreover, integration of our atlas with data from other species will allow for a better understanding of vertebrate retinal evolution.
KW  - Animals
KW  - Sharks: genetics
KW  - Retina: metabolism
KW  - Retina: cytology
KW  - Female
KW  - Sequence Analysis, RNA
KW  - Single-Cell Analysis
LB  - PUB:(DE-HGF)16
C6  - pmid:39920165
C2  - pmc:PMC11806052
DO  - DOI:10.1038/s41597-025-04547-2
UR  - https://inrepo02.dkfz.de/record/298613
ER  -