TY  - JOUR
AU  - Rajagopal, Varshni
AU  - Seiler, Jeanette Simone
AU  - Nasa, Isha
AU  - Cantarella, Simona
AU  - Theiss, Jana
AU  - Herget, Franziska
AU  - Kaifer, Bianca
AU  - Klostermann, Melina
AU  - Will, Rainer
AU  - Schneider, Martin
AU  - Helm, Dominic
AU  - König, Julian
AU  - Zarnack, Kathi
AU  - Diederichs, Sven
AU  - Kettenbach, Arminja N
AU  - Caudron-Herger, Maiwen
TI  - An atlas of RNA-dependent proteins in cell division reveals the riboregulation of mitotic protein-protein interactions.
JO  - Nature Communications
VL  - 16
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Springer Nature
M1  - DKFZ-2025-00518
SP  - 2325
PY  - 2025
N1  - #EA:B150#LA:B150#
AB  - Ribonucleoprotein complexes are dynamic assemblies of RNA with RNA-binding proteins, which modulate the fate of RNA. Inversely, RNA riboregulates the interactions and functions of the associated proteins. Dysregulation of ribonucleoprotein functions is linked to diseases such as cancer and neurological disorders. In dividing cells, RNA and RNA-binding proteins are present in mitotic structures, but their impact on cell division remains unclear. By applying the proteome-wide R-DeeP strategy to cells synchronized in mitosis versus interphase integrated with the RBP2GO knowledge, we provided an atlas of RNA-dependent proteins in cell division, accessible at R-DeeP3.dkfz.de. We uncovered AURKA, KIFC1 and TPX2 as unconventional RNA-binding proteins. KIFC1 was identified as a new substrate of AURKA, and new TPX2-interacting protein. Their pair-wise interactions were RNA dependent. In addition, RNA stimulated AURKA kinase activity and stabilized its conformation. In this work, we highlighted riboregulation of major mitotic factors as an additional complexity level of cell division.
KW  - Humans
KW  - Aurora Kinase A: metabolism
KW  - Aurora Kinase A: genetics
KW  - Mitosis
KW  - Cell Cycle Proteins: metabolism
KW  - Cell Cycle Proteins: genetics
KW  - Microtubule-Associated Proteins: metabolism
KW  - Microtubule-Associated Proteins: genetics
KW  - RNA-Binding Proteins: metabolism
KW  - RNA-Binding Proteins: genetics
KW  - HeLa Cells
KW  - RNA: metabolism
KW  - RNA: genetics
KW  - Protein Binding
KW  - Cell Division
KW  - Nuclear Proteins: metabolism
KW  - Nuclear Proteins: genetics
KW  - Proteome: metabolism
KW  - Aurora Kinase A (NLM Chemicals)
KW  - Cell Cycle Proteins (NLM Chemicals)
KW  - Microtubule-Associated Proteins (NLM Chemicals)
KW  - TPX2 protein, human (NLM Chemicals)
KW  - RNA-Binding Proteins (NLM Chemicals)
KW  - RNA (NLM Chemicals)
KW  - AURKA protein, human (NLM Chemicals)
KW  - Nuclear Proteins (NLM Chemicals)
KW  - Proteome (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40057470
C2  - pmc:PMC11890761
DO  - DOI:10.1038/s41467-025-57671-3
UR  - https://inrepo02.dkfz.de/record/299577
ER  -