Journal Article

DKFZ-2025-00518

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png An atlas of RNA-dependent proteins in cell division reveals the riboregulation of mitotic protein-protein interactions.

Rajagopal, V. (First author)DKFZ* ; Seiler, J. S. (First author)DKFZ* ; Nasa, I. ; Cantarella, S.DKFZ* ; Theiss, J.DKFZ* ; Herget, F.DKFZ* ; Kaifer, B.DKFZ* ; Klostermann, M. ; Will, R.DKFZ* ; Schneider, M.DKFZ* ; Helm, D.DKFZ* ; König, J. ; Zarnack, K. ; Diederichs, S.DKFZ* ; Kettenbach, A. N. ; Caudron-Herger, M. (Last author)DKFZ*

2025
Springer Nature [London]

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Please use a persistent id in citations: doi:10.1038/s41467-025-57671-3

Abstract: Ribonucleoprotein complexes are dynamic assemblies of RNA with RNA-binding proteins, which modulate the fate of RNA. Inversely, RNA riboregulates the interactions and functions of the associated proteins. Dysregulation of ribonucleoprotein functions is linked to diseases such as cancer and neurological disorders. In dividing cells, RNA and RNA-binding proteins are present in mitotic structures, but their impact on cell division remains unclear. By applying the proteome-wide R-DeeP strategy to cells synchronized in mitosis versus interphase integrated with the RBP2GO knowledge, we provided an atlas of RNA-dependent proteins in cell division, accessible at R-DeeP3.dkfz.de. We uncovered AURKA, KIFC1 and TPX2 as unconventional RNA-binding proteins. KIFC1 was identified as a new substrate of AURKA, and new TPX2-interacting protein. Their pair-wise interactions were RNA dependent. In addition, RNA stimulated AURKA kinase activity and stabilized its conformation. In this work, we highlighted riboregulation of major mitotic factors as an additional complexity level of cell division.

Keyword(s): Humans (MeSH) ; Aurora Kinase A: metabolism (MeSH) ; Aurora Kinase A: genetics (MeSH) ; Mitosis (MeSH) ; Cell Cycle Proteins: metabolism (MeSH) ; Cell Cycle Proteins: genetics (MeSH) ; Microtubule-Associated Proteins: metabolism (MeSH) ; Microtubule-Associated Proteins: genetics (MeSH) ; RNA-Binding Proteins: metabolism (MeSH) ; RNA-Binding Proteins: genetics (MeSH) ; HeLa Cells (MeSH) ; RNA: metabolism (MeSH) ; RNA: genetics (MeSH) ; Protein Binding (MeSH) ; Cell Division (MeSH) ; Nuclear Proteins: metabolism (MeSH) ; Nuclear Proteins: genetics (MeSH) ; Proteome: metabolism (MeSH) ; Aurora Kinase A ; Cell Cycle Proteins ; Microtubule-Associated Proteins ; TPX2 protein, human ; RNA-Binding Proteins ; RNA ; AURKA protein, human ; Nuclear Proteins ; Proteome

Note: #EA:B150#LA:B150#

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Contributing Institute(s):

1. B150 Molekulare RNA Biologie und Krebs (B150)
2. Zelluläre Tools (W111)
3. Proteomics (W120)
4. DKTK Koordinierungsstelle Freiburg (FR01)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2025

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