TY  - JOUR
AU  - LaBelle, Jenna J
AU  - Haase, Rebecca D
AU  - Beck, Alexander
AU  - Haase, Jacob
AU  - Jiang, Li
AU  - Oliveira de Biagi, Carlos Alberto
AU  - Neyazi, Sina
AU  - Englinger, Bernhard
AU  - Liu, Ilon
AU  - Trissal, Maria
AU  - Jeong, Daeun
AU  - Hack, Olivia A
AU  - Nascimento, Andrezza
AU  - Shaw, McKenzie L
AU  - Nguyen, Cuong M
AU  - Castellani, Sophia
AU  - Mathewson, Nathan D
AU  - Ashenberg, Orr
AU  - Veiga Cruzeiro, Gustavo Alencastro
AU  - Rosenberg, Tom
AU  - Vogelzang, Jayne R
AU  - Pyrdol, Jason
AU  - Marx, Sascha
AU  - Luomo, Adrienne M
AU  - Godicelj, Anze
AU  - Baumgartner, Alicia
AU  - Rozowsky, Jacob S
AU  - Madlener, Sibylle
AU  - Mayr, Lisa
AU  - Peyrl, Andreas
AU  - Geyeregger, Rene
AU  - Loetsch, Daniela
AU  - Dorfer, Christian
AU  - Haberler, Christine
AU  - Stepien, Natalia
AU  - Slavc, Irene
AU  - Davidson, Tom Belle
AU  - Prins, Robert M
AU  - Yeo, Kee Kiat
AU  - Cooney, Tabitha
AU  - Ligon, Keith
AU  - Lidov, Hart
AU  - Alexandrescu, Sanda
AU  - Baird, Lissa C
AU  - Gojo, Johannes
AU  - Wucherpfennig, Kai W
AU  - Filbin, Mariella G
TI  - Dissecting the immune landscape in pediatric high-grade glioma reveals cell state changes under therapeutic pressure.
JO  - Cell reports / Medicine
VL  - 6
IS  - 5
SN  - 2666-3791
CY  - Cambridge, MA
PB  - Cell Press
M1  - DKFZ-2025-00920
SP  - 102095
PY  - 2025
N1  - Volume 6, Issue 5, 20 May 2025, 102095
AB  - Pediatric high-grade gliomas (pHGGs) are among the most lethal childhood tumors. While therapeutic approaches were largely adapted from adult treatment regime, significant biological differences between pediatric and adult gliomas exist, which influence the immune microenvironment and may contribute to the limited response to current pHGG treatment strategies. We provide a comprehensive transcriptomic analysis of the pHGG immune landscape using single-cell RNA sequencing and spatial transcriptomics. We analyze matched malignant, myeloid, and T cells from patients with pediatric diffuse high-grade glioma (HGG) or high-grade ependymoma, examining immune microenvironment distinctions after chemo-/radiotherapy, immune checkpoint inhibition treatment, and by age. Our analysis reveals differences in the proportions of pediatric myeloid subpopulations compared to adult counterparts. Additionally, we observe significant shifts toward immune-suppressive environments following cancer therapy. Our findings offer valuable insights into potential immunotherapy targets and serve as a robust resource for understanding immune microenvironmental variations across HGG age groups and treatment regimens.
KW  - immuno-oncology (Other)
KW  - pediatric high-grade glioma (Other)
KW  - single-cell RNA-seq (Other)
KW  - tumor microenvironment (Other)
KW  - tumor-infiltrating immune cell biology (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40315846
DO  - DOI:10.1016/j.xcrm.2025.102095
UR  - https://inrepo02.dkfz.de/record/300772
ER  -