Journal Article

DKFZ-2025-00920

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dissecting the immune landscape in pediatric high-grade glioma reveals cell state changes under therapeutic pressure.

LaBelle, J. J. ; Haase, R. D. ; Beck, A. ; Haase, J. ; Jiang, L. ; Oliveira de Biagi, C. A. ; Neyazi, S. ; Englinger, B. ; Liu, I. ; Trissal, M. ; Jeong, D. ; Hack, O. A. ; Nascimento, A. ; Shaw, M. L. ; Nguyen, C. M. ; Castellani, S. ; Mathewson, N. D. ; Ashenberg, O. ; Veiga Cruzeiro, G. A. ; Rosenberg, T. ; Vogelzang, J. R. ; Pyrdol, J. ; Marx, S. ; Luomo, A. M. ; Godicelj, A. ; Baumgartner, A. ; Rozowsky, J. S. ; Madlener, S. ; Mayr, L. ; Peyrl, A. ; Geyeregger, R. ; Loetsch, D. ; Dorfer, C. ; Haberler, C. ; Stepien, N. ; Slavc, I. ; Davidson, T. B. ; Prins, R. M. ; Yeo, K. K. ; Cooney, T. ; Ligon, K. ; Lidov, H. ; Alexandrescu, S. ; Baird, L. C. ; Gojo, J.DKFZ* ; Wucherpfennig, K. W. ; Filbin, M. G.

2025
Cell Press Cambridge, MA

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Please use a persistent id in citations: doi:10.1016/j.xcrm.2025.102095

Abstract: Pediatric high-grade gliomas (pHGGs) are among the most lethal childhood tumors. While therapeutic approaches were largely adapted from adult treatment regime, significant biological differences between pediatric and adult gliomas exist, which influence the immune microenvironment and may contribute to the limited response to current pHGG treatment strategies. We provide a comprehensive transcriptomic analysis of the pHGG immune landscape using single-cell RNA sequencing and spatial transcriptomics. We analyze matched malignant, myeloid, and T cells from patients with pediatric diffuse high-grade glioma (HGG) or high-grade ependymoma, examining immune microenvironment distinctions after chemo-/radiotherapy, immune checkpoint inhibition treatment, and by age. Our analysis reveals differences in the proportions of pediatric myeloid subpopulations compared to adult counterparts. Additionally, we observe significant shifts toward immune-suppressive environments following cancer therapy. Our findings offer valuable insights into potential immunotherapy targets and serve as a robust resource for understanding immune microenvironmental variations across HGG age groups and treatment regimens.

Keyword(s): immuno-oncology ; pediatric high-grade glioma ; single-cell RNA-seq ; tumor microenvironment ; tumor-infiltrating immune cell biology

Note: Volume 6, Issue 5, 20 May 2025, 102095

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Contributing Institute(s):

1. B062 Pädiatrische Neuroonkologie (B062)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2025

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