TY  - JOUR
AU  - Perçin, Gülce
AU  - Riege, Konstantin
AU  - Fröbel, Julia
AU  - Metz, Jonas
AU  - Culemann, Stephan
AU  - Lesche, Mathias
AU  - Reinhardt, Susanne
AU  - Höfer, Thomas
AU  - Hoffmann, Steve
AU  - Waskow, Claudia
TI  - Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool.
JO  - Nature Communications
VL  - 16
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Springer Nature
M1  - DKFZ-2025-00988
SP  - 4428
PY  - 2025
AB  - Embryonic macrophages emerge before the onset of definitive hematopoiesis, seed into discrete tissues and contribute to specialized resident macrophages throughout life. Presence of embryonic macrophages in the bone marrow and functional impact on hematopoietic stem cells (HSC) or the niche remains unclear. Here we show that bone marrow macrophages consist of two ontogenetically distinct cell populations from embryonic and adult origin. Newborn mice lacking embryonic macrophages have decreased HSC numbers in the bone marrow suggesting an important function for embryo-derived macrophages in orchestrating HSC trafficking around birth. The establishment of a normal cellular niche space in the bone marrow critically depends on embryonic macrophages that are important for the development of mesenchymal stromal cells, but not other non-hematopoietic niche cells, providing evidence for a specific role for embryo-derived macrophages in the establishment of the niche environment pivotal for the establishment of a normally sized HSC pool.
KW  - Animals
KW  - Hematopoietic Stem Cells: cytology
KW  - Hematopoietic Stem Cells: physiology
KW  - Macrophages: cytology
KW  - Macrophages: metabolism
KW  - Macrophages: physiology
KW  - Stem Cell Niche: physiology
KW  - Mice
KW  - Homeostasis
KW  - Hematopoiesis
KW  - Mice, Inbred C57BL
KW  - Embryo, Mammalian: cytology
KW  - Mesenchymal Stem Cells: cytology
KW  - Animals, Newborn
KW  - Female
KW  - Bone Marrow Cells: cytology
LB  - PUB:(DE-HGF)16
C6  - pmid:40368907
DO  - DOI:10.1038/s41467-025-59059-9
UR  - https://inrepo02.dkfz.de/record/301316
ER  -