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@ARTICLE{Lund:301747,
      author       = {S. Lund and C. Gong and X. Yu and L. M. Staudt and D. J.
                      Hodson and S. Scheich$^*$},
      title        = {{S}trategies for {CRISPR}-based knock-ins in primary human
                      {B} cells and lymphoma cell lines.},
      journal      = {Frontiers in immunology},
      volume       = {16},
      issn         = {1664-3224},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {DKFZ-2025-01131},
      pages        = {1589729},
      year         = {2025},
      abstract     = {Since its advent about ten years ago, the CRISPR-Cas9
                      system has been frequently used in biomedical applications.
                      It has advanced various fields, and CRISPR-Cas9-based
                      therapeutics have shown promising results in the treatment
                      of specific hematological diseases. Furthermore, CRISPR gene
                      editing technologies have revolutionized cancer research by
                      enabling a broad range of genetic perturbations, including
                      genetic knockouts and precise single nucleotide changes.
                      This perspective focuses on the state-of-the-art methodology
                      of CRISPR knock-ins to engineer immune cells. Since this
                      technique relies on homology-directed repair (HDR) of
                      double-strand breaks (DSBs) induced by the Cas9 enzyme, it
                      can be used to introduce specific mutations into the target
                      genome. Therefore, this methodology offers a valuable
                      opportunity to functionally study specific mutations and to
                      uncover their impacts not only on overall cell functions but
                      also on the mechanisms behind cancer-related alterations in
                      common signaling pathways. This article highlights CRISPR
                      knock-in strategies, protocols, and applications in cancer
                      and immune research, with a focus on diffuse large B cell
                      lymphoma.},
      subtyp        = {Review Article},
      keywords     = {Humans / CRISPR-Cas Systems / Gene Knock-In Techniques:
                      methods / Gene Editing: methods / B-Lymphocytes: metabolism
                      / B-Lymphocytes: immunology / Cell Line, Tumor / Lymphoma,
                      Large B-Cell, Diffuse: genetics / DLBCL (Other) / NF-kappa B
                      (NF-KB) (Other) / gene editing (CRISPR/Cas9) (Other) /
                      knock-in (Other) / lymphoma (Other)},
      cin          = {FM01},
      ddc          = {610},
      cid          = {I:(DE-He78)FM01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40453079},
      pmc          = {pmc:PMC12122508},
      doi          = {10.3389/fimmu.2025.1589729},
      url          = {https://inrepo02.dkfz.de/record/301747},
}