guest ::
| Home > Publications database > Strategies for CRISPR-based knock-ins in primary human B cells and lymphoma cell lines. |
| Home > Publications database > Strategies for CRISPR-based knock-ins in primary human B cells and lymphoma cell lines. |
| Journal Article (Review Article) | DKFZ-2025-01131 |
; ; ; ; ;
2025
Frontiers Media
Lausanne
This record in other databases: 
Please use a persistent id in citations: doi:10.3389/fimmu.2025.1589729
Abstract: Since its advent about ten years ago, the CRISPR-Cas9 system has been frequently used in biomedical applications. It has advanced various fields, and CRISPR-Cas9-based therapeutics have shown promising results in the treatment of specific hematological diseases. Furthermore, CRISPR gene editing technologies have revolutionized cancer research by enabling a broad range of genetic perturbations, including genetic knockouts and precise single nucleotide changes. This perspective focuses on the state-of-the-art methodology of CRISPR knock-ins to engineer immune cells. Since this technique relies on homology-directed repair (HDR) of double-strand breaks (DSBs) induced by the Cas9 enzyme, it can be used to introduce specific mutations into the target genome. Therefore, this methodology offers a valuable opportunity to functionally study specific mutations and to uncover their impacts not only on overall cell functions but also on the mechanisms behind cancer-related alterations in common signaling pathways. This article highlights CRISPR knock-in strategies, protocols, and applications in cancer and immune research, with a focus on diffuse large B cell lymphoma.
Keyword(s): Humans (MeSH) ; CRISPR-Cas Systems (MeSH) ; Gene Knock-In Techniques: methods (MeSH) ; Gene Editing: methods (MeSH) ; B-Lymphocytes: metabolism (MeSH) ; B-Lymphocytes: immunology (MeSH) ; Cell Line, Tumor (MeSH) ; Lymphoma, Large B-Cell, Diffuse: genetics (MeSH) ; DLBCL ; NF-kappa B (NF-KB) ; gene editing (CRISPR/Cas9) ; knock-in ; lymphoma
; 
; 
; Article Processing Charges ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection
|
The record appears in these collections: |
