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@ARTICLE{Braun:301907,
      author       = {L. M. Braun$^*$ and R. Zeiser$^*$},
      title        = {{P}rotocol to study in vivo organ-specific migration of
                      apoptotic splenocytes in mice with tumor and immune
                      checkpoint inhibitor-induced colitis.},
      journal      = {STAR Protocols},
      volume       = {6},
      number       = {2},
      issn         = {2666-1667},
      address      = {Cambridge, MA},
      publisher    = {Cell Press},
      reportid     = {DKFZ-2025-01177},
      pages        = {103878},
      year         = {2025},
      note         = {Protocol},
      abstract     = {Extracorporeal photopheresis (ECP) reduces immune
                      checkpoint inhibitor (ICI)-induced colitis without affecting
                      anti-tumor immunity. Here, we provide a protocol to trace
                      ECP-treated cells in vivo after transfer into tumor-bearing
                      mice with ICI-induced colitis. We detail tumor and colitis
                      induction, ICI therapy, and the labeling of ECP-treated
                      splenocytes to trace their migration into target organs.
                      Fluorescence imaging and tissue digestion enable the
                      analysis of phagocytic uptake and the analysis of
                      tolerogenic polarization of phagocytes based on congenic
                      markers of donor and recipient mice. For complete details on
                      the use and execution of this protocol, please refer to
                      Braun et al.1.},
      keywords     = {cancer (Other) / immunology (Other) / model organisms
                      (Other)},
      cin          = {FR01},
      ddc          = {600},
      cid          = {I:(DE-He78)FR01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40482036},
      doi          = {10.1016/j.xpro.2025.103878},
      url          = {https://inrepo02.dkfz.de/record/301907},
}