Journal Article

DKFZ-2025-01177

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protocol to study in vivo organ-specific migration of apoptotic splenocytes in mice with tumor and immune checkpoint inhibitor-induced colitis.

Braun, L. M.DKFZ* ; Zeiser, R.DKFZ*

2025
Cell Press Cambridge, MA

Abstract: Extracorporeal photopheresis (ECP) reduces immune checkpoint inhibitor (ICI)-induced colitis without affecting anti-tumor immunity. Here, we provide a protocol to trace ECP-treated cells in vivo after transfer into tumor-bearing mice with ICI-induced colitis. We detail tumor and colitis induction, ICI therapy, and the labeling of ECP-treated splenocytes to trace their migration into target organs. Fluorescence imaging and tissue digestion enable the analysis of phagocytic uptake and the analysis of tolerogenic polarization of phagocytes based on congenic markers of donor and recipient mice. For complete details on the use and execution of this protocol, please refer to Braun et al.1.

Keyword(s): cancer ; immunology ; model organisms

Note: Protocol

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Contributing Institute(s):

1. DKTK Koordinierungsstelle Freiburg (FR01)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2025

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Database coverage:
; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Emerging Sources Citation Index ; Fees ; SCOPUS ; Web of Science Core Collection

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