Journal Article

DKFZ-2025-01239

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protocol to study phagocytosis of apoptotic murine splenocytes by syngeneic antigen-presenting cells in vitro.

Braun, L. M.DKFZ* ; Zeiser, R.DKFZ*

2025
Cell Press Cambridge, MA

Abstract: Extracorporeal photopheresis (ECP) effectively reduces immune checkpoint inhibitor (ICI)-induced colitis and is based on the phagocytic uptake of apoptotic ECP-treated splenocytes. Here, we present a flow cytometry-based assay to investigate phagocytosis of ECP-treated murine splenocytes by bone marrow-derived macrophages and dendritic cells in vitro using a congenic marker only present in donor animals and fluorescence labeling of ECP-treated cells. We describe steps for analyzing M2-like polarization markers, investigating protein phosphorylation, and analyzing specific target gene expression in single cells. For complete details on the use and execution of this protocol, please refer to Braun et al.1.

Keyword(s): Cancer ; Immunology ; Microscopy

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Contributing Institute(s):

1. DKTK Koordinierungsstelle Freiburg (FR01)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2025

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Database coverage:
; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Emerging Sources Citation Index ; Fees ; SCOPUS ; Web of Science Core Collection

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