%0 Journal Article
%A Yuan, Tanwei
%A Tagscherer, Katrin E
%A Roth, Wilfried
%A Bewerunge-Hudler, Melanie
%A Brobeil, Alexander
%A Kloor, Matthias
%A Bläker, Hendrik
%A Brenner, Hermann
%A Hoffmeister, Michael
%T Methylation-based smoking signatures in blood and tissue samples for the prediction of self-reported smoking status and mortality in patients with colorectal cancer.
%J Clinical epigenetics
%V 17
%N 1
%@ 1868-7075
%C [Erscheinungsort nicht ermittelbar]
%I BioMed Central
%M DKFZ-2025-01350
%P 113
%D 2025
%Z #EA:C070#LA:C070#
%X Smoking is a well-established risk factor for colorectal cancer (CRC) development. However, the reliability of DNA methylation-based smoking signatures in predicting smoking status and their prognostic value in CRC remain unclear, particularly across different biological sample types.Five previously validated methylation-based smoking signatures were analyzed in 2237 CRC patients with blood-derived DNA and 2273 patients with tumor tissue-derived DNA. Blood-derived signatures showed strong correlations with self-reported smoking status, effectively differentiating current smokers from never smokers (all p < 0.0001), with excellent discriminative ability (median area under the receiver operating characteristic curve: 0.94). In contrast, tumor tissue-derived signatures exhibited much weaker associations with smoking status. Among non-metastatic CRC patients, blood-derived methylation signatures were significantly associated with increased risks of all-cause and non-CRC-related mortality, but not with CRC-specific mortality. Conversely, two tumor tissue-derived signatures demonstrated stronger associations with CRC-specific mortality compared to blood-derived signatures.Blood-derived methylation-based smoking signatures are robust indicators for smoking exposure and are associated with increased mortality risk among non-metastatic CRC patients. When applied to tumor tissue, signatures showed stronger associations with CRC-specific mortality.
%K Humans
%K Colorectal Neoplasms: genetics
%K Colorectal Neoplasms: mortality
%K Colorectal Neoplasms: blood
%K DNA Methylation
%K Male
%K Female
%K Middle Aged
%K Smoking: genetics
%K Smoking: adverse effects
%K Smoking: blood
%K Self Report
%K Aged
%K Prognosis
%K Risk Factors
%K Colorectal cancer (Other)
%K DNA methylation biomarkers (Other)
%K Prognosis (Other)
%K Smoking (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40611182
%2 pmc:PMC12225191
%R 10.1186/s13148-025-01918-9
%U https://inrepo02.dkfz.de/record/302810