Journal Article

DKFZ-2025-01508

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.

Ditzer, N. ; Senoglu, E. ; Kolodziejczyk, A. ; Schütze, T. M. ; Nikolaidi, A. ; Küster, K. ; Sameith, K. ; Dietz, S. ; Derihaci, R. P. ; Birdir, C. ; Eugster, A. ; Karl, M. O. ; Dahl, A. ; Wimberger, P. ; Baenke, F.DKFZ* ; Peitzsch, C. ; Albert, M.

2025
Cell Press [Cambridge, Mass.]

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Please use a persistent id in citations: doi:10.1016/j.neuron.2025.06.016

Abstract: Epigenetic mechanisms regulate gene expression programs during neurogenesis, but the extent of epigenetic remodeling during human cortical development remains unknown. Here, we characterize the epigenetic landscape of the human developing neocortex by leveraging Epi-CyTOF, a mass-cytometry-based approach for the simultaneous single-cell analysis of more than 30 epigenetic marks. We identify Polycomb repressive complex 2 (PRC2)-mediated H3K27me3 as the modification with the strongest cell-type-specific enrichment. Inhibition of PRC2 in human cortical organoids resulted in a shift of neural progenitor cell (NPC) proliferation toward differentiation. Cell-type-specific profiling of H3K27me3 identified neuronal differentiation and extracellular matrix (ECM) genes in the human neocortex. PRC2 inhibition resulted in increased production of the ECM proteins Syndecan 1 and laminin alpha 1. Overall, this study comprehensively characterizes the epigenetic state of specific neural cell types and highlights a novel role for H3K27me3 in regulating the ECM composition in the human developing neocortex.

Keyword(s): Polycomb repressive complex 2 ; brain development ; extracellular matrix ; gene expression ; histone methylation ; human cortical organoid ; human fetal cortex ; neural stem cell ; neurogenesis ; single cell

Note: Volume 113, Issue 18, 17 September 2025, Pages 2927-2944.e10

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Contributing Institute(s):

1. DKTK Koordinierungsstelle Dresden (DD01)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2025

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 15 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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