TY  - JOUR
AU  - Geisse, Antonia
AU  - Zhang, Tao
AU  - Schreiber, Jonathan
AU  - Markova, Kristina
AU  - Burkhalter, Sophie
AU  - Wardemann, Hedda
AU  - Macpherson, Andrew J
AU  - Rollenske, Tim
TI  - Efficient Expression and Purification of Recombinant Mouse Dimeric IgA.
JO  - European journal of immunology
VL  - 55
IS  - 9
SN  - 0014-2980
CY  - Weinheim
PB  - Wiley-VCH
M1  - DKFZ-2025-01921
SP  - e70055
PY  - 2025
AB  - Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.
KW  - Animals
KW  - Mice
KW  - Immunoglobulin A: isolation & purification
KW  - Immunoglobulin A: genetics
KW  - Immunoglobulin A: immunology
KW  - Immunoglobulin A: biosynthesis
KW  - Recombinant Proteins: isolation & purification
KW  - Recombinant Proteins: genetics
KW  - Recombinant Proteins: immunology
KW  - B-Lymphocytes: immunology
KW  - Antibodies, Monoclonal: isolation & purification
KW  - Antibodies, Monoclonal: genetics
KW  - Antibodies, Monoclonal: immunology
KW  - Protein Multimerization
KW  - J chain (Other)
KW  - dimeric IgA (Other)
KW  - immunoglobulin A (Other)
KW  - mucosal B cell responses (Other)
KW  - recombinant monoclonal antibody (Other)
KW  - Immunoglobulin A (NLM Chemicals)
KW  - Recombinant Proteins (NLM Chemicals)
KW  - Antibodies, Monoclonal (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40952320
C2  - pmc:PMC12435132
DO  - DOI:10.1002/eji.70055
UR  - https://inrepo02.dkfz.de/record/304602
ER  -