Journal Article

DKFZ-2025-01921

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Efficient Expression and Purification of Recombinant Mouse Dimeric IgA.

Geisse, A. ; Zhang, T. ; Schreiber, J. ; Markova, K. ; Burkhalter, S. ; Wardemann, H.DKFZ* ; Macpherson, A. J. ; Rollenske, T.

2025
Wiley-VCH Weinheim

Abstract: Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.

Keyword(s): Animals (MeSH) ; Mice (MeSH) ; Immunoglobulin A: isolation & purification (MeSH) ; Immunoglobulin A: genetics (MeSH) ; Immunoglobulin A: immunology (MeSH) ; Immunoglobulin A: biosynthesis (MeSH) ; Recombinant Proteins: isolation & purification (MeSH) ; Recombinant Proteins: genetics (MeSH) ; Recombinant Proteins: immunology (MeSH) ; B-Lymphocytes: immunology (MeSH) ; Antibodies, Monoclonal: isolation & purification (MeSH) ; Antibodies, Monoclonal: genetics (MeSH) ; Antibodies, Monoclonal: immunology (MeSH) ; Protein Multimerization (MeSH) ; J chain ; dimeric IgA ; immunoglobulin A ; mucosal B cell responses ; recombinant monoclonal antibody ; Immunoglobulin A ; Recombinant Proteins ; Antibodies, Monoclonal

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Contributing Institute(s):

1. B-Zell-Immunologie (D130)

Research Program(s):

1. 314 - Immunologie und Krebs (POF4-314) (POF4-314)

Appears in the scientific report 2025

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Database coverage:
; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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