%0 Journal Article
%A Brinkert, Pia
%A Krebs, Lena
%A Samperio Ventayol, Pilar
%A Greune, Lilo
%A Bannach, Carina
%A Amakiri, Cynthia
%A Bucher, Delia
%A Kollasser, Jana
%A Dersch, Petra
%A Boulant, Steeve
%A Stradal, Theresia E B
%A Schelhaas, Mario
%T The actin nucleation promoting factor WASH facilitates clathrin-independent endocytosis of human papillomaviruses.
%J EMBO reports
%V 26
%N 22
%@ 1469-221X
%C [London]
%I Nature Publishing Group UK
%M DKFZ-2025-02606
%P 5533 - 5566
%D 2025
%X Endocytosis is a fundamental cellular process facilitated by diverse mechanisms. Remarkably, several distinct clathrin-independent endocytic processes have been identified and characterized following virus uptake into cells. For some, however, mechanistic execution and biological function remain largely unclear. This includes an endocytic process exploited by human papillomavirus type 16 (HPV16). Using HPV16, we examine how vesicles are formed by combining systematic cellular perturbations with electron and video microscopy. Cargo uptake occurs by uncoated, inward-budding pits. Mechanistically, vesicle scission is facilitated by actin polymerization controlled through the actin nucleation-promoting factor WASH. While WASH typically functions in conjunction with the retromer complex on endosomes during retrograde trafficking, endocytic vesicle formation is largely independent of retromer itself and the heterodimeric membrane-bending SNX-BAR retromer adaptor, thereby uncovering a role of WASH in endocytosis in addition to its canonical role in intracellular membrane trafficking.
%K Endocytosis
%K Humans
%K Clathrin: metabolism
%K Actins: metabolism
%K Human papillomavirus 16: physiology
%K Microfilament Proteins: metabolism
%K Microfilament Proteins: genetics
%K Endosomes: metabolism
%K Virus Internalization
%K HeLa Cells
%K Papillomavirus Infections: virology
%K Papillomavirus Infections: metabolism
%K Human Papillomavirus Viruses
%K Actin (Other)
%K Endocytosis (Other)
%K Nucleation Promoting Factor (Other)
%K Virus Entry (Other)
%K WASH (Other)
%K Clathrin (NLM Chemicals)
%K Actins (NLM Chemicals)
%K Microfilament Proteins (NLM Chemicals)
%K WASH protein, human (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41073792
%2 pmc:PMC12635285
%R 10.1038/s44319-025-00594-3
%U https://inrepo02.dkfz.de/record/306541