Journal Article

DKFZ-2025-02606

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The actin nucleation promoting factor WASH facilitates clathrin-independent endocytosis of human papillomaviruses.

Brinkert, P. ; Krebs, L. ; Samperio Ventayol, P. ; Greune, L. ; Bannach, C. ; Amakiri, C. ; Bucher, D. ; Kollasser, J. ; Dersch, P. ; Boulant, S.DKFZ* ; Stradal, T. E. B. ; Schelhaas, M.

2025
Nature Publishing Group UK [London]

Abstract: Endocytosis is a fundamental cellular process facilitated by diverse mechanisms. Remarkably, several distinct clathrin-independent endocytic processes have been identified and characterized following virus uptake into cells. For some, however, mechanistic execution and biological function remain largely unclear. This includes an endocytic process exploited by human papillomavirus type 16 (HPV16). Using HPV16, we examine how vesicles are formed by combining systematic cellular perturbations with electron and video microscopy. Cargo uptake occurs by uncoated, inward-budding pits. Mechanistically, vesicle scission is facilitated by actin polymerization controlled through the actin nucleation-promoting factor WASH. While WASH typically functions in conjunction with the retromer complex on endosomes during retrograde trafficking, endocytic vesicle formation is largely independent of retromer itself and the heterodimeric membrane-bending SNX-BAR retromer adaptor, thereby uncovering a role of WASH in endocytosis in addition to its canonical role in intracellular membrane trafficking.

Keyword(s): Endocytosis (MeSH) ; Humans (MeSH) ; Clathrin: metabolism (MeSH) ; Actins: metabolism (MeSH) ; Human papillomavirus 16: physiology (MeSH) ; Microfilament Proteins: metabolism (MeSH) ; Microfilament Proteins: genetics (MeSH) ; Endosomes: metabolism (MeSH) ; Virus Internalization (MeSH) ; HeLa Cells (MeSH) ; Papillomavirus Infections: virology (MeSH) ; Papillomavirus Infections: metabolism (MeSH) ; Human Papillomavirus Viruses (MeSH) ; Actin ; Endocytosis ; Nucleation Promoting Factor ; Virus Entry ; WASH ; Clathrin ; Actins ; Microfilament Proteins ; WASH protein, human

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Contributing Institute(s):

1. NWG Infection and Immune Sensing Dynamics (F140)

Research Program(s):

1. 319H - Addenda (POF4-319H) (POF4-319H)

Appears in the scientific report 2025

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Database coverage:
; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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