TY  - JOUR
AU  - Brinkert, Pia
AU  - Krebs, Lena
AU  - Samperio Ventayol, Pilar
AU  - Greune, Lilo
AU  - Bannach, Carina
AU  - Amakiri, Cynthia
AU  - Bucher, Delia
AU  - Kollasser, Jana
AU  - Dersch, Petra
AU  - Boulant, Steeve
AU  - Stradal, Theresia E B
AU  - Schelhaas, Mario
TI  - The actin nucleation promoting factor WASH facilitates clathrin-independent endocytosis of human papillomaviruses.
JO  - EMBO reports
VL  - 26
IS  - 22
SN  - 1469-221X
CY  - [London]
PB  - Nature Publishing Group UK
M1  - DKFZ-2025-02606
SP  - 5533 - 5566
PY  - 2025
AB  - Endocytosis is a fundamental cellular process facilitated by diverse mechanisms. Remarkably, several distinct clathrin-independent endocytic processes have been identified and characterized following virus uptake into cells. For some, however, mechanistic execution and biological function remain largely unclear. This includes an endocytic process exploited by human papillomavirus type 16 (HPV16). Using HPV16, we examine how vesicles are formed by combining systematic cellular perturbations with electron and video microscopy. Cargo uptake occurs by uncoated, inward-budding pits. Mechanistically, vesicle scission is facilitated by actin polymerization controlled through the actin nucleation-promoting factor WASH. While WASH typically functions in conjunction with the retromer complex on endosomes during retrograde trafficking, endocytic vesicle formation is largely independent of retromer itself and the heterodimeric membrane-bending SNX-BAR retromer adaptor, thereby uncovering a role of WASH in endocytosis in addition to its canonical role in intracellular membrane trafficking.
KW  - Endocytosis
KW  - Humans
KW  - Clathrin: metabolism
KW  - Actins: metabolism
KW  - Human papillomavirus 16: physiology
KW  - Microfilament Proteins: metabolism
KW  - Microfilament Proteins: genetics
KW  - Endosomes: metabolism
KW  - Virus Internalization
KW  - HeLa Cells
KW  - Papillomavirus Infections: virology
KW  - Papillomavirus Infections: metabolism
KW  - Human Papillomavirus Viruses
KW  - Actin (Other)
KW  - Endocytosis (Other)
KW  - Nucleation Promoting Factor (Other)
KW  - Virus Entry (Other)
KW  - WASH (Other)
KW  - Clathrin (NLM Chemicals)
KW  - Actins (NLM Chemicals)
KW  - Microfilament Proteins (NLM Chemicals)
KW  - WASH protein, human (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:41073792
C2  - pmc:PMC12635285
DO  - DOI:10.1038/s44319-025-00594-3
UR  - https://inrepo02.dkfz.de/record/306541
ER  -