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| Home > Publications database > Single-Cell Hyperthermia: Diamond Quantum Thermometry Reveals Thermal Control of Macrophage Polarization. |
| Home > Publications database > Single-Cell Hyperthermia: Diamond Quantum Thermometry Reveals Thermal Control of Macrophage Polarization. |
| Journal Article | DKFZ-2025-02836 |
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2025
Wiley-VCH
Weinheim
Abstract: Fever elevates body temperature to enhance immune response; however, intracellular temperature can fluctuate by up to 15 °C, suggesting a previously unrecognized layer of thermal regulation. While hyperthermia has long been exploited in medicine, how localized temperature gradients influence cellular fate remains poorly understood. Here, a dual-function nanodiamond platform is introduced that integrates optically detected magnetic resonance (ODMR) thermometry with croconium-dye-based photothermal heating to precisely modulate temperature within endo-lysosomal compartments of macrophages. Controlled intracellular hyperthermia triggers oxidative stress, transcriptional reprogramming, and polarization toward a pro-inflammatory phenotype, as confirmed by immunofluorescence, flow cytometry, and transcriptomics. These findings reveal intracellular thermal gradients as active regulators of immune signaling and gene expression. By establishing a direct subcellular thermal trigger for immune activation, independent of the canonical heat-shock pathway. This work introduces a quantum-enabled strategy for probing and programming cellular thermodynamics at the nanoscale.
Keyword(s): fluorescent nanodiamond ; immune response modulation ; intracellular sensing ; lysosomal temperature regulation
; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; DEAL Wiley ; Essential Science Indicators ; IF >= 25 ; JCR ; Nationallizenz
; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection
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