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| Home > Publications database > CLL cell-derived soluble factors do not influence the functionality of normal B cells. |
| Journal Article | DKFZ-2026-01319 |
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2026
Frontiers Media
Lausanne
Abstract: Humoral immunodeficiency is frequently observed in patients affected by chronic lymphocytic leukemia (CLL). The reasons for the compromised B-cell responses are only partly understood. We hypothesized that the normal residual B cells in CLL patients are negatively influenced in their functionality by soluble factors produced by CLL cells.We performed functional B-cell assays to analyze the influence of serum from CLL patients or conditioned medium from CLL cells cultivated with stromal cells on B cells from healthy donors. Serum from healthy individuals or conditioned medium from normal B cells was used as controls, respectively. We stimulated the B cells in a T-cell-dependent fashion and incubated them in the respective serum or conditioned medium for 5 days. We measured plasma cell differentiation, proliferation, cell death, and B-cell activation, the latter using the markers CD80, CD86, and CD25.We did not detect significant differences supporting the hypothesis that CLL-derived soluble factors negatively influence the proliferation, activation, or cell death levels of normal B cells from healthy individuals in in vitro coculture.Our analysis suggests no major direct inhibitory effects of CLL-derived soluble factors on normal mature B cells. Thus, it is likely that other factors lead to the known B-cell dysfunction in CLL.
Keyword(s): Humans (MeSH) ; Leukemia, Lymphocytic, Chronic, B-Cell: immunology (MeSH) ; Leukemia, Lymphocytic, Chronic, B-Cell: blood (MeSH) ; Leukemia, Lymphocytic, Chronic, B-Cell: metabolism (MeSH) ; Leukemia, Lymphocytic, Chronic, B-Cell: pathology (MeSH) ; B-Lymphocytes: immunology (MeSH) ; B-Lymphocytes: metabolism (MeSH) ; Lymphocyte Activation: immunology (MeSH) ; Cell Proliferation (MeSH) ; Coculture Techniques (MeSH) ; Culture Media, Conditioned (MeSH) ; Cell Differentiation (MeSH) ; Female (MeSH) ; Male (MeSH) ; Aged (MeSH) ; CLL ; activation ; functionality ; leukemia ; normal residual B cells ; proliferation ; Culture Media, Conditioned
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