Journal Article

DKFZ-2026-01900

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protocol for single-cell epigenetic profiling in human organoids and tumoroids with Epi-CyTOF.

Senoglu, E. ; Mittné, V. ; Dietz, S. ; Eugster, A. ; Thieme, S. ; Baenke, F.DKFZ* ; Albert, M. ; Peitzsch, C.

2026
Cell Press Cambridge, MA

Abstract: In this protocol, we present Epi-CyTOF, a cytometry by time of flight (CyTOF) approach for single-cell epigenome profiling in cortical organoids as a model system for human brain development. We describe steps for in silico panel design, antibody conjugation and testing, sample preparation and staining, and high-dimensional data analysis. Additionally, we provide recommendations for applying Epi-CyTOF to other model systems based on our experience with gastroesophageal tumoroids. For complete details on the use and execution of this protocol, please refer to Ditzer et al .1.

Keyword(s): Bioinformatics ; Genomics ; Immunology ; Neuroscience ; cancer ; cell biology ; developmental biology ; mass cytometry ; molecular biology ; organoids ; single cell

Note: #DKTKZFB26# / #NCTZFB26#

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Contributing Institute(s):

1. DKTK Koordinierungsstelle Dresden (DD01)
2. Koordinierungsstelle NCT Dresden (DD04)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2026

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Database coverage:
; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Emerging Sources Citation Index ; Fees ; SCOPUS ; Web of Science Core Collection

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