Journal Article

DKFZ-2026-02163

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Age- and sex-dependent heterogeneity during LPS-induced murine acute lung injury.

Crispens, C. ; Wilken-Schmitz, A. ; Dern, V. ; Rahimi, A. R. ; Madau, E. ; Frigger, N. C. ; Jakob, J. ; Häupl, B.DKFZ* ; Oellerich, T.DKFZ* ; Weigert, A. ; Talbot, S. R. ; Zacharowski, K. ; Heinicke, U. ; von Knethen, A.

2026
BioMed Central London

Abstract: Acute respiratory distress syndrome (ARDS) remains difficult to treat due to its heterogeneous etiology. In this study, a mouse model of intratracheal lipopolysaccharide (LPS) instillation was used to investigate age- and sex-dependent immune responses. After six hours of LPS exposure and one hour of mechanical ventilation, immune cell subtypes as well as mRNA and protein expression were analyzed. Aged mice exhibited a basal pro-inflammatory immune signature already under sham conditions, whereas young mice displayed a more balanced response with parallel pro- and anti-inflammatory mechanisms. Notably, aged female LPS treated mice exhibited an exclusively pro-inflammatory response. UMAP analysis revealed distinct clustering according to age, sex, and treatment, while aged male mice formed isolated clusters. These findings highlight the importance of considering age- and sex-specific therapeutic strategies for ARDS treatment.

Keyword(s): ALI ; ARDS ; Ageing ; Immune response ; Inflammation ; MICU ; Mouse model ; Sex- and age-specific differences

Note: #DKTKZFB26#

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Contributing Institute(s):

1. DKTK Koordinierungsstelle Frankfurt (FM01)
2. DKTK FM Proteomics Facility (FM03)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2026

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Database coverage:
; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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