guest ::
| Home > Publications database > A cell-based model system links chromothripsis with hyperploidy. |
| Home > Publications database > A cell-based model system links chromothripsis with hyperploidy. |
| Journal Article | DKFZ-2017-03090 |
; ; ; ; ; ; ; ; ; ; ; ; ; ;
2015
EMBO Press
Heidelberg
This record in other databases: 
Abstract: A remarkable observation emerging from recent cancer genome analyses is the identification of chromothripsis as a one-off genomic catastrophe, resulting in massive somatic DNA structural rearrangements (SRs). Largely due to lack of suitable model systems, the mechanistic basis of chromothripsis has remained elusive. We developed an integrative method termed 'complex alterations after selection and transformation (CAST),' enabling efficient in vitro generation of complex DNA rearrangements including chromothripsis, using cell perturbations coupled with a strong selection barrier followed by massively parallel sequencing. We employed this methodology to characterize catastrophic SR formation processes, their temporal sequence, and their impact on gene expression and cell division. Our in vitro system uncovered a propensity of chromothripsis to occur in cells with damaged telomeres, and in particular in hyperploid cells. Analysis of primary medulloblastoma cancer genomes verified the link between hyperploidy and chromothripsis in vivo. CAST provides the foundation for mechanistic dissection of complex DNA rearrangement processes.
Keyword(s): Telomeric Repeat Binding Protein 2
; 
; 
; BIOSIS Previews ; Current Contents - Life Sciences ; DOAJ Seal ; Ebsco Academic Search ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection
|
The record appears in these collections: |
