Journal Article

DKFZ-2017-03729

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.

Vicuña, L. ; Strochlic, D. E. ; Latremoliere, A. ; Bali, K. K. ; Simonetti, M. ; Husainie, D. ; Prokosch, S.DKFZ* ; Riva, P. ; Griffin, R. S. ; Njoo, C. ; Gehrig, S. ; Mall, M. A. ; Arnold, B.DKFZ* ; Devor, M. ; Woolf, C. J. ; Liberles, S. D. ; Costigan, M. ; Kuner, R.

2015
Nature America Inc. New York, NY

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Please use a persistent id in citations: doi:10.1038/nm.3852

Abstract: Neuropathic pain is a major, intractable clinical problem and its pathophysiology is not well understood. Although recent gene expression profiling studies have enabled the identification of novel targets for pain therapy, classical study designs provide unclear results owing to the differential expression of hundreds of genes across sham and nerve-injured groups, which can be difficult to validate, particularly with respect to the specificity of pain modulation. To circumvent this, we used two outbred lines of rats, which are genetically similar except for being genetically segregated as a result of selective breeding for differences in neuropathic pain hypersensitivity. SerpinA3N, a serine protease inhibitor, was upregulated in the dorsal root ganglia (DRG) after nerve injury, which was further validated for its mouse homolog. Mice lacking SerpinA3N developed more neuropathic mechanical allodynia than wild-type (WT) mice, and exogenous delivery of SerpinA3N attenuated mechanical allodynia in WT mice. T lymphocytes infiltrate the DRG after nerve injury and release leukocyte elastase (LE), which was inhibited by SerpinA3N derived from DRG neurons. Genetic loss of LE or exogenous application of a LE inhibitor (Sivelastat) in WT mice attenuated neuropathic mechanical allodynia. Overall, we reveal a novel and clinically relevant role for a member of the serpin superfamily and a leukocyte elastase and crosstalk between neurons and T cells in the modulation of neuropathic pain.

Keyword(s): Acute-Phase Proteins ; Enzyme Inhibitors ; Serpina3n protein, mouse ; Serpina3n protein, rat ; Serpins ; Leukocyte Elastase

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Contributing Institute(s):

1. Molekulare Immunologie (D050)

Research Program(s):

1. 314 - Tumor immunology (POF3-314) (POF3-314)

Appears in the scientific report 2015

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Database coverage:
; BIOSIS Previews ; Current Contents - Clinical Medicine ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 30 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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