Journal Article

DKFZ-2018-02210

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry.

Bärenz, F. (First author)DKFZ* ; Kschonsak, Y. T.DKFZ* ; Meyer, A.DKFZ* ; Jafarpour, A. ; Lorenz, H. ; Hoffmann, I. (Last author)DKFZ*

2018
American Society for Cell Biology Bethesda, Md.

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Please use a persistent id in citations: doi:10.1091/mbc.E18-02-0115

Abstract: Microtubule nucleation was uncovered as a key principle of spindle assembly. However, the mechanistic details about microtubule nucleation and the organization of spindle formation and symmetry are currently being revealed. Here we describe the function of Coiled-coil domain containing 61 (Ccdc61), a so far uncharacterized centrosomal protein, in spindle assembly and symmetry. Our data describe that Ccdc61 is required for spindle assembly and precise chromosome alignments in mitosis. Microtubule tip-tracking experiments in the absence of Ccdc61 reveal a clear loss of the intrinsic symmetry of microtubule tracks within the spindle. Furthermore, we show that Ccdc61 controls the centrosomal localization of Cep170, a protein that was shown previously to localize to centrosomes as well as spindle microtubules and promotes microtubule organization and microtubule assembly. Interestingly, selective disruption of Ccdc61 impairs the binding between Cep170 and TBK1, an interaction that is required for microtubule stability. In summary, we have discovered Ccdc61 as a centrosomal protein with an important function in mitotic microtubule organization. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].

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Contributing Institute(s):

1. Zellzykluskontrolle und Carcinogenese (F045)

Research Program(s):

1. 316 - Infections and cancer (POF3-316) (POF3-316)

Appears in the scientific report 2018

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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