Journal Article

DKFZ-2019-02850

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Transcriptional profiling of medulloblastoma with extensive nodularity (MBEN) reveals two clinically relevant tumor subsets with VSNL1 as potent prognostic marker.

Korshunov, A. (First author)DKFZ* ; Okonechnikov, K.DKFZ* ; Sahm, F.DKFZ* ; Ryzhova, M. ; Stichel, D.DKFZ* ; Schrimpf, D.DKFZ* ; Ghasemi, D. R.DKFZ* ; Pajtler, K. W.DKFZ* ; Antonelli, M. ; Donofrio, V. ; Mastronuzzi, A. ; Rossi, S. ; Camassei, F. D. ; Buccoliero, A. M. ; Haberler, C. ; Slavc, I. ; Dahiya, S. ; Casalini, B.DKFZ* ; Sievers, P.DKFZ* ; Meyer, J.DKFZ* ; Kumirova, E. ; Zheludkova, O. ; Golanov, A. ; Jones, D. T. W.DKFZ* ; Pfister, S. M.DKFZ* ; Kool, M.DKFZ* ; von Deimling, A. (Last author)DKFZ*

2020
Springer Heidelberg

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Please use a persistent id in citations: doi:10.1007/s00401-019-02102-z

Abstract: Medulloblastoma with extensive nodularity (MBEN) is one of the few central nervous system (CNS) tumor entities occurring in infants which is traditionally associated with good to excellent prognosis. Some MBEN, however, have been reported with an unfavorable clinical course. We performed an integrated DNA/RNA-based molecular analysis of a multi-institutional MBEN cohort (n = 41) to identify molecular events which might be responsible for variability in patients' clinical outcomes. RNA sequencing analysis of this MBEN cohort disclosed two clear transcriptome clusters (TCL) of these CNS tumors: 'TCL1 MBEN' and 'TCL2 MBEN' which were associated with various gene expression signatures, mutational landscapes and, importantly, prognosis. Thus, the clinically unfavorable 'TCL1 MBEN' subset revealed transcriptome signatures composed of cancer-associated signaling pathways and disclosed a high frequency of clinically relevant germline PTCH1/SUFU alterations. In contrast, gene expression profiles of tumors from the clinically favorable 'TCL2 MBEN' subgroup were associated with activation of various neurometabolic and neurotransmission signaling pathways, and germline SHH-pathway gene mutations were extremely rare in this transcriptome cluster. 'TCL2 MBEN' also revealed strong and ubiquitous expression of VSNL1 (visinin-like protein 1) both at the mRNA and protein level, which was correlated with a favorable clinical course. Thus, combining mutational and epigenetic profiling with transcriptome analysis including VSNL1 immunohistochemistry, MBEN patients could be stratified into clinical risk groups of potential value for subsequent treatment planning.

Note: 2020 Mar;139(3):583-596.#EA:B300#LA:B300#

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Contributing Institute(s):

1. KKE Neuropathologie (B300)
2. B062 Pädiatrische Neuroonkologie (B062)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2020

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 15 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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