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| Home > Publications database > Influence of photon, proton and carbon ion irradiation on differentiation, maturation and functionality of dendritic cells. |
| Home > Publications database > Influence of photon, proton and carbon ion irradiation on differentiation, maturation and functionality of dendritic cells. |
| Journal Article | DKFZ-2022-00527 |
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2022
Tampa, Fla.
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Please use a persistent id in citations: doi:10.31083/j.fbs1401002
Abstract: While the primary purpose of radiotherapy (RT) is the elimination of cancer cells by inducing DNA-damage, considerable evidence emerges that anti-neoplastic effects extend beyond mere tumor cell killing. These secondary effects are based on activation of dendritic cells (DCs) via induction of antitumoral immune reactions. However, there is an ongoing debate whether or not irradiation of the DCs themselves may negatively affect their maturation and functionality. Human monocytes were irradiated with different absorbed doses (1 × 15 Gy relative biological effectiveness (RBE), 5 × 2 Gy (RBE), 1 × 0.5 Gy (RBE)) with photons, protons and carbon ions. Differentiation and maturation of DCs were assessed by staining of corresponding cell surface molecules and functional analysis of irradiated DCs was based on in vitro analysis of phagocytosis, migration and IL-12 secretion. Irradiation of CD14-positive DCs did not alter surface phenotypes of immature DCs and mature DCs. Not only differentiation, but also functionality of immature DCs regarding phagocytosis, migration and IL-12 secretion capacity was not negatively influenced through RT with photons, protons or carbon ions as well as with different dose levels. After proton irradiation migratory capacity of immature DCs was increased. Our experiments reveal that phenotypic maturation of DCs remains unchanged after RT with different fractionations and after irradiation with particle therapy. Unaffected functionality (phagocytosis, migration and cytokine secretion) after RT of DCs indicated possible persistent potential for inducing adaptive immune response. Additional effects on the immunogenic potential of DCs will be investigated by further functional assays.
Keyword(s): Carbon ion ; Dendritic cells ; Maturation ; Monocyte derived ; Monocytes ; Particle therapy ; Phagocytosis ; Protons ; Radioimmunology ; Radiotherapy
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