Journal Article

DKFZ-2022-01212

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSO.

Casati, B. (First author)DKFZ* ; Verdi, J. P.DKFZ* ; Hempelmann, A.DKFZ* ; Kittel, M. ; Klaebisch, A. G. ; Meister, B. ; Welker, S. ; Asthana, S.DKFZ* ; Di Giorgio, S.DKFZ* ; Boskovic, P.DKFZ* ; Man, K. H.DKFZ* ; Schopp, M.DKFZ* ; Ginno, P. A.DKFZ* ; Radlwimmer, B.DKFZ* ; Stebbins, C. E.DKFZ* ; Miethke, T. ; Papavasiliou, F. (Last author)DKFZ* ; Pecori, R. (Last author)DKFZ*

2022
Nature Publishing Group UK [London]

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Please use a persistent id in citations: doi:10.1038/s41467-022-30862-y

Abstract: During the ongoing COVID-19 pandemic, PCR testing and antigen tests have proven critical for helping to stem the spread of its causative agent, SARS-CoV-2. However, these methods suffer from either general applicability and/or sensitivity. Moreover, the emergence of variant strains creates the need for flexibility to correctly and efficiently diagnose the presence of substrains. To address these needs we developed the diagnostic test ADESSO (Accurate Detection of Evolving SARS-CoV-2 through SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing) Optimization) which employs Cas13 to diagnose patients in 1 h without sophisticated equipment. Using an extensive panel of clinical samples, we demonstrate that ADESSO correctly identifies infected individuals at a sensitivity and specificity comparable to RT-qPCR on extracted RNA and higher than antigen tests for unextracted samples. Altogether, ADESSO is a fast, sensitive and cheap method that can be applied in a point of care setting to diagnose COVID-19 and can be quickly adjusted to detect new variants.

Note: These authors jointly supervised this work: Thomas Miethke, Fotini Nina Papavasiliou, Riccardo Pecori. ✉email: Thomas.Miethke@medma.uni-heidelberg.de; n.papavasiliou@dkfz.de; r.pecori@dkfz.de#EA:D150#LA:D150#

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Contributing Institute(s):

1. D150 Immundiversität (D150)
2. D160 Strukturbiologie von Infektion und Immunität (D160)
3. B060 Molekulare Genetik (B060)
4. B270 Regulatorische Genomik und Evolution von Tumoren (B270)

Research Program(s):

1. 314 - Immunologie und Krebs (POF4-314) (POF4-314)

Appears in the scientific report 2022

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